In vivo tracking of toxic diesel particulate matter in mice using radiolabeling and nuclear imaging

Abstract

Exposure to diesel particulate matter (DPM) is associated with several adverse health effects, including severe respiratory diseases. Quantitative analysis of DPM in vivo can provide important information on the behavior of harmful chemicals, as well as their toxicological impacts in living subjects. This study presents whole-body images and tissue distributions of DPM in animal models, using molecular imaging and radiolabeling techniques. The self-assembly of the ⁸⁹Zr-labeled pyrene analog with a suspension of DPM efficiently produced ⁸⁹Zr-incorporated DPM (⁸⁹Zr-DPM). Positron emission tomography images were obtained for mice exposed to ⁸⁹Zr-DPM via three administration routes: intratracheal, oral, and intravenous injection. DPM was largely distributed in the lungs and only slowly cleared after 7 days in mice exposed via the intratracheal route. In addition, a portion of ⁸⁹Zr-DPM was translocated to other organs, such as the heart, spleen, and liver. Uptake values in these organs were also noticeable following exposure via the intravenous route. In contrast, most of the orally administered DPM was excreted quickly within a day. These results suggest that continuous inhalation exposure to DPM causes serious lung damage and may cause toxic effects in the extrapulmonary organs.

Keywords: Biodistribution; Diesel particulate matter; Positron emission tomography imaging; Radiolabeling; Toxicological study.