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. 2022 Dec 1;23(12):4177-4183.
doi: 10.31557/APJCP.2022.23.12.4177.

Immunoexpression of Tumor Infiltrating Lymphocytes(TILS) CD4 + and CD8 + in Oral Squamous Cell Carcinoma (OSCC) in Correlations with Clinicopathological Characteristics and Prognosis

Hanan Salman Wahbi  1 , Ahmad Manadili  1
Affiliations

Immunoexpression of Tumor Infiltrating Lymphocytes(TILS) CD4 + and CD8 + in Oral Squamous Cell Carcinoma (OSCC) in Correlations with Clinicopathological Characteristics and Prognosis

Hanan Salman Wahbi et al. Asian Pac J Cancer Prev. .

Abstract

Objective: This study aimed to analyze CD4 +and CD8 + TILs in oral squamous cell carcinoma (OSCC) and to correlate it with histologic grade of malignancy and clinicopathologic data.

Methods: The sample was composed of 43 archived specimens. Clinical features and histological grade of malignancy were obtained. The infiltrating intensity of CD4 +, CD8 positive cells were assessed by immunohistochemistry. One-way ANOVA was used to study the association between CD4 +, CD8 + and the grade of OSCC. The cut-off values of the proposed diagnostic indices were received from calculating the coordinates of the receiver operating characteristic (ROC) curve. For clinicopathologic data Independent-Samples T test, Pearson Correlation Coefficient, Correlation Coefficient were used clinicopathologic characteristics.

Results: CD4 +and CD8 + were observed in all specimens. CD4 + were more frequent in poorly differentiated specimens (74.14) (P= 0.021<0.05). CD8 + were more frequent in well- differentiated specimens (51.18). None of these correlations were significant (P=0.454>0.05). CD4 +/ CD8 ratio was higher in low-grade specimens (180.28) (P=0.017<0.05). No differences between CD4 +, CD8 +and CD4 +/ CD8 ratio between poorly- differentiated and moderately- differentiated groups ROC P value (0.370, 0.248, 0.126) respectively. there is a difference between CD4 +, CD4 +/ CD8ratio between poorly- differentiated and well- differentiated groups ROC P value (0.022, 0.341, 0.012) Sensitivity (0.857, 0.882), specificity (0.706, 0.857) respectively. and no differences between CD8 + poorly- differentiated and well- differentiated groups ROC P value (0.341). there is a difference between CD4 + between moderately - differentiated and well- differentiated groups ROC P value (0.038) Sensitivity (0.368), specificity (0,765). No significant correlation was obtained with clinicopathologic findings of OSCC.

Conclusion: CD4 + and CD4 +/ CD8 + ratio are independent prognostic factor in OSCC.

Keywords: CD4 +; CD8 +; OSCC; Prognosis; TILs.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
Expression of cd4+ and cd8+ based on IHC Cell Count Analysis in Well–Differentiated OSCCA( H&E) Staining the Tumor Cells Nests (the blue arrow ) and the immune cells) ,B the infiltration of cd4+ ( the yellow arrow) in stroma off OSCC between tumor nests ,C the infiltration of cd8+ ( the yellow arrow) in stroma of OSCC between tumor nests
Figure 2
Figure 2
Expression of cd4+ and cd8+ based on IHC Cell Count Analysis in Moderetly – Differentiated OSCCA(H&E) staining the tumor cells nests (the blue arrow ) and the immune cells), B the infiltration of cd4+ ( the yellow arrow) in stroma off oscc between tumor nests ,C the infiltration of cd8+ ( the yellow arrow) in stroma of OSCC between tumor nests
Figure 3
Figure 3
Expression of cd4+ and cd8+ based on IHC Cell Count Analysis in Poorly – Differentiated OSCCA( H&E) Staining the Tumor Cells Nests (the blue arrow ) and the immune cells) ,B the infiltration of cd4+ ( the yellow arrow) in stroma of OSCC between tumor nests ,C the infiltration of cd8+ ( the yellow arrow) in stroma of OSCC between tumor nests
Figure 4
Figure 4
Represent the Mean of CD4+(A) CD8+(B)and CD4+/CD8+(C) in the Histological Grades of OSCC
Figure 5
Figure 5
Represent the ROC Curve(A) represent the ROC Curve between CD4+ and the incidence OSCC (the blue line) in the groups of well-differentiated and poorly-differentiated>. OSCC (the blue line) in the groups of well-differentiated and poorly-differentiated>. (B) represent the ROC Curve between CD4+/CD8+ and the incidence of OSCC (the blue line) in the groups of well-differentiated and poorly-differentiated>>
Figure 6
Figure 6
Represent the ROC Curve of CD4+ and the Incidence OSCC (the Blue Line) in the Groups of Well-Differentiated and Moderately-Differentiated
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