Water acidification weakens the carbon sink capacity of mixotrophic organisms

Abstract

Mixotrophs combine both autotrophic and heterotrophic cell structures, and their highly plastic nutritional modes can shape the structure of food web and affect the carbon sink capacity of aquatic ecosystems. As pH affects the growth of phytoplankton by altering the carbonate balance system, water acidification caused by environmental pollution and global climate change may affect the nutritional modes of mixotrophs and bring a serious environmental consequence. In this study, we cultured mixotrophic Ochromonas gloeopara under autotrophic, mixotrophic, and heterotrophic conditions at different pH levels to test the tendency of its nutritional model and the changes in photosynthetic carbon fixation capacity. Results showed that: (1) with decreasing pH, carbon uptake of Ochromonas through phagocytosis gradually replaced the carbon fixation of photosynthesis; (2) with increasing pH, Ochromonas grazing rate decreased, and the relative contribution of photosynthetic carbon fixation to total carbon acquisition increased for Ochromonas; (3) Ochromonas became more heterotrophic under water acidification, which was involved in the up-regulated expression of genes encoding key enzymes that regulate nutrient perception, movement ability, and cell repair. These findings suggested that acidification caused mixotrophic organisms to become more heterotrophic, which can change their functional role and weaken their carbon sink capacity.

Keywords: Carbon sink; Mixotrophy; Nutritional strategy; Photosynthesis; Water acidification.