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. 2022 Nov-Dec;26(6):539-543.
doi: 10.4103/jisp.jisp_453_21. Epub 2022 Nov 14.

Comparative evaluation of the efficacy of a novel tetracycline-coated suture with triclosan-coated and noncoated sutures on bacterial load reduction: A prospective in vitro study

Akshaya Bhupesh Banodkar  1 , Vaibhavi Pandurang Nandgaonkar  1 , Rajesh Prabhakar Gaikwad  1 , Lynette Custodio Fernandes  1 , Chitra Laxmikant Patil  1 , Amrita Dharmendra Batho  1
Affiliations

Comparative evaluation of the efficacy of a novel tetracycline-coated suture with triclosan-coated and noncoated sutures on bacterial load reduction: A prospective in vitro study

Akshaya Bhupesh Banodkar et al. J Indian Soc Periodontol. 2022 Nov-Dec.

Abstract

Background: Surgical site infection (SSI) is one of the common postoperative complications observed after various periodontal surgeries, and sutures play a vital role in its causation. Thus, the aim of this study is to evaluate and compare the efficacy of a novel tetracycline-coated suture with triclosan-coated and nonantibacterial-coated sutures on bacterial load reduction to prevent SSI by measuring the zone of inhibition.

Materials and methods: Twenty systemically healthy individuals with moderate chronic periodontitis were included in this study. Fresh unstimulated saliva was collected from each patient and inoculated on three different blood agar plates. Sutures were divided into three groups (Group A: Tetracycline-coated suture, Group B: Triclosan-coated suture, Group C [control group]: Nonantibacterial-coated suture). The antibacterial efficacy of each suture was evaluated by performing agar diffusion test. The zone of inhibition around each suture was calculated, and statistical analysis was performed for the same using Kruskall-Wallis ANOVA test and Mann-Whitney U-test.

Results: On intergroup comparison, there was a statistically highly significant difference seen for the zone of inhibition between the groups (P < 0.01) with the highest values in Group A (14.45 mm), followed by Group B (1.4 mm) and least in Group C (0 mm).

Conclusion: Tetracycline-coated suture is more efficacious than triclosan-coated suture to reduce bacterial load and further prevent SSIs. However, in vivo clinical trial is must to prove the same.

Keywords: Agar diffusion test; surgical site infection; suture; tetracycline-coated suture; triclosan-coated suture; zone of inhibition.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Flow chart of study design. n – number of samples
Figure 2
Figure 2
Procedure for preparation of tetracycline-coated sutures. (a) Armamentarium showing from left to right 1% sodium hydroxide, 2% aqueous acetic acid, 2 g sodium alginate polymer, 8 g chitosan and, undyed vicryl suture; (b and c) Scouring of suture; (d and e) Preparation of chitosan solution; (f) Placement of suture into the chitosan solution; (g) Twenty pieces of suture of 3 cm length; (h) Placement of suture threads into tetracycline solution; (i) Tetracycline-coated sutures
Figure 3
Figure 3
Zone of inhibition around sutures. (a) Zone of inhibition around tetracycline-coated suture; (b) Zone of inhibition around triclosan-coated suture; (c) Zone of inhibition around nonantibacterial-coated suture)
Figure 4
Figure 4
Intergroup comparison of zone of inhibition
See this image and copyright information in PMC

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