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. 2023 May;195(5):3295-3310.
doi: 10.1007/s12010-022-04294-9. Epub 2022 Dec 31.

Biodegradation of the Pesticides Bifenthrin and Fipronil by Bacillus Isolated from Orange Leaves

Juliana Galan Viana  1 , Willian Garcia Birolli  2 , André Luiz Meleiro Porto  3
Affiliations

Biodegradation of the Pesticides Bifenthrin and Fipronil by Bacillus Isolated from Orange Leaves

Juliana Galan Viana et al. Appl Biochem Biotechnol. 2023 May.

Abstract

The pyrethroid bifenthrin and the phenylpyrazole fipronil are widely employed insecticides, and their extensive use became an environmental issue. Therefore, this study evaluated their biodegradation employing bacterial strains of Bacillus species isolated from leaves of orange trees, aiming at new biocatalysts with high efficiency for use singly and in consortium. Experiments were performed in liquid culture medium at controlled temperature and stirring (32 °C, 130 rpm). After 5 days, residual quantification by HPLC-UV/Vis showed that Bacillus amyloliquefaciens RFD1C presented 93% biodegradation of fipronil (10.0 mg.L-1 initial concentration) and UPLC-HRMS analyses identified the metabolite fipronil sulfone. Moreover, Bacillus pseudomycoides 3RF2C showed a biodegradation of 88% bifenthrin (30.0 mg.L-1 initial concentration). A consortium composed of the 8 isolated strains biodegraded 81% fipronil and 51% bifenthrin, showing that this approach did not promote better results than the most efficient strains employed singly, although high rates of biodegradation were observed. In conclusion, bacteria of the Bacillus genus isolated from leaves of citrus biodegraded these pesticides widely applied to crops, showing the importance of the plant microbiome for degradation of toxic xenobiotics.

Keywords: Bacteria; Bioremediation; Biotransformation; Fipronil sulfone; Insecticide; Pyrethroid.

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