Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1987 Sep 25;15(18):7235-48.
doi: 10.1093/nar/15.18.7235.

Solid phase synthesis of oligoribonucleotides using the o-nitrobenzyl group for 2'-hydroxyl protection and H-phosphonate chemistry

T Tanaka  1 , S Tamatsukuri, M Ikehara
Affiliations
Free PMC article
Comparative Study

Solid phase synthesis of oligoribonucleotides using the o-nitrobenzyl group for 2'-hydroxyl protection and H-phosphonate chemistry

T Tanaka et al. Nucleic Acids Res. .
Free PMC article

Abstract

Oligoribonucleotides with chain length of 7, 11, 15, 17, 24 and 34 were synthesized on long chain alkylamine controlled pore glass beads (LCA-CPG) using o-nitrobenzyl protection of 2'-hydroxyls via a H-phosphonate approach either manually or by using an automatic synthesizer. The oligoribonucleotides were obtained in yields of 0.6 0.6-20%, based on initial nucleoside bound to the LCA-CPG support.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1974 Mar;1(3):331-53 - PubMed
    1. Nucleic Acids Res. 1981 Apr 10;9(7):1691-706 - PubMed
    1. Nucleic Acids Res. 1982 Mar 11;10(5):1755-69 - PubMed
    1. Nucleic Acids Res. 1986 Aug 11;14(15):6265-79 - PubMed
    1. Nucleic Acids Res. 1985 May 24;13(10):3651-66 - PubMed

Publication types

Substances