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. 2023:2600:45-62.
doi: 10.1007/978-1-0716-2851-5_3.

Quantification of Apparent Membrane Tension and Membrane-to-Cortex Attachment in Animal Cells Using Atomic Force Microscopy-Based Force Spectroscopy

Martin Bergert  1 , Alba Diz-Muñoz  2
Affiliations

Quantification of Apparent Membrane Tension and Membrane-to-Cortex Attachment in Animal Cells Using Atomic Force Microscopy-Based Force Spectroscopy

Martin Bergert et al. Methods Mol Biol. 2023.

Abstract

Reliable quantification of a cell's biophysical properties is key for understanding the role of mechanics in cell biology. Plasma membrane tension, the energetic cost of increasing the surface area of the plasma membrane, has been shown to regulate a plethora of cellular processes, ranging from leading edge formation to phagocytosis and membrane trafficking. Here, we describe the measurement of this key mechanical property of the cell surface using atomic force microscopy (AFM)-based force spectroscopy. Depending on the nature of the force curve acquisition, AFM measurements can quantify various membrane tension components, such as apparent membrane tension and membrane-to-cortex attachment (MCA). We discuss the biophysical background (1), required materials (2), sample preparation (3.1), AFM-probe calibration and functionalization (3.2), force curve acquisition (3.3) and data analysis and representation (3.4).

Keywords: AFM; Atomic force microscopy; Atomic force spectroscopy; Cantilever; MCA; Membrane tension; Membrane-to-cortex attachment; Tether.

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References

    1. Diz-Muñoz A, Weiner OD, Fletcher DA (2018) In pursuit of the mechanics that shape cell surfaces. Nat Phys 14(7):648–652 - DOI
    1. Diz-Muñoz A, Fletcher DA, Weiner OD (2013) Use the force: membrane tension as an organizer of cell shape and motility. Trends Cell Biol 23(2):47–53 - DOI
    1. Salbreux G, Charras G, Paluch E (2012) Actin cortex mechanics and cellular morphogenesis. Trends Cell Biol 22(10):536–545 - DOI
    1. Kelkar M, Bohec P, Charras G (2020) Mechanics of the cellular actin cortex: from signalling to shape change. Curr Opin Cell Biol 66:69–78 - DOI
    1. Pontes B, Viana NB, Salgado LT, Farina M, Moura Neto V, Nussenzveig HM (2011) Cell cytoskeleton and tether extraction. Biophys J 101(1):43–52 - DOI

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