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. 2021 Dec 24;37(3):417-423.
doi: 10.46497/ArchRheumatol.2022.9217. eCollection 2022 Sep.

Dysregulation of complement factor H in juvenile-onset systemic lupus erythematosus patients

Eman Eissa  1 , Botros Morcos  1 , Dalia Dorgham  2 , Naglaa Kholoussi  1
Affiliations

Dysregulation of complement factor H in juvenile-onset systemic lupus erythematosus patients

Eman Eissa et al. Arch Rheumatol. .

Abstract

Objectives: This study aims to evaluate the expression pattern of factor H in peripheral blood and the frequency of factor H autoantibodies in plasma of juvenile-onset systemic lupus erythematosus (jSLE) patients compared to healthy controls.

Patients and methods: Between March 2019 and October 2019, a total of 30 healthy individuals (3 males, 27 females; mean age: 26±7.4 years; range, 18 to 40 years) and 65 jSLE patients (age of onset ≤16 years) (2 males, 63 females; mean age: 23.4±7 years; range, 15 to 38 years) were included. Factor H expression pattern was examined in blood of all subjects using quantitative real-time polymerase chain reaction and the frequency of factor H autoantibodies was estimated in plasma using enzyme-linked immunosorbent assay.

Results: Factor H expression was significantly downregulated in jSLE patients compared to healthy controls (p<0.01). A significant underexpression of factor H was observed in jSLE patients with nephritis compared to those without nephritis (p<0.03), while there was no association of factor H expression levels with any of the other clinical and serological features, disease activity or disease damage index of patients. Only 5% of jSLE patients were positive for factor H autoantibodies without any correlations with the clinical data or disease activity of patients.

Conclusion: Our study results suggest that factor H expression can be dysregulated in jSLE patients.

Keywords: Complement factor H; factor H autoantibodies; juvenile-onset systemic lupus erythematosus; nephritis..

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Conflict of interest statement

Conflict of Interest: The authors declared no conflicts of interest with respect to the authorship and/or publication of this article.

Figures

Figure 1
Figure 1. (a) Fold change of factor H in jSLE patients relative to normal controls. Bar graph represents median of fold change. (b) Frequency of factor H autoantibodies in jSLE patients. * Statistically significant at p<0.01 versus controls (by Mann Whitney U test); jSLE: Juvenile-onset systemic lupus erythematosus.
See this image and copyright information in PMC

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