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. 2023:2606:159-170.
doi: 10.1007/978-1-0716-2879-9_12.

The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)

Dong Yang  1   2   3   4 , Chun-Qing Song  5   6   7   8
Affiliations

The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)

Dong Yang et al. Methods Mol Biol. 2023.

Abstract

A genetic disorder is a disease caused by an abnormal DNA sequence, and almost half of the known pathogenic monogenetic mutations are caused by G-to-A mutation (Landrum et al., Nucleic Acids Res 44:D862-868, 2016). Adenine base editors (ABE), developed from the CRISPR, hold the great promise to mediate the A-to-G transition in genomic DNA while not inducing DNA cleavage (Gaudelli et al., Nature 551:464-471, 2017). Additionally, lipid nanoparticles (LNPs), as a non-viral delivery, are able to deliver the ABE mRNAs and gRNA to the target tissues (Newby and Liu, Mol Ther 29:3107-3124, 2021). This chapter mainly introduces the production and LNP delivery of ABE mRNA and gRNA.

Keywords: ABE mRNA; Delivery; LNPs; Murine liver.

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