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. 2022 Dec 29:15:2925-2932.
doi: 10.2147/CCID.S388418. eCollection 2022.

Anthocyanin from Lycium ruthenicum Murr. in the Qaidam Basin Alleviates Ultraviolet-Induced Apoptosis of Human Skin Fibroblasts by Regulating the Death Receptor Pathway

Liwen Wang #  1 , Guangmei Wan #  1 , Gang Wang  1 , Meihong Zhang  1 , Nanxin Li  1 , Qinning Zhang  2 , Hualing Yan  1
Affiliations

Anthocyanin from Lycium ruthenicum Murr. in the Qaidam Basin Alleviates Ultraviolet-Induced Apoptosis of Human Skin Fibroblasts by Regulating the Death Receptor Pathway

Liwen Wang et al. Clin Cosmet Investig Dermatol. .

Abstract

Purpose: The study aimed to investigate the potential protective role of anthocyanin from Lycium ruthenicum Murr. in the Qaidam Basin against ultraviolet B (UVB)-induced apoptosis of human skin fibroblasts (HSFs).

Methods: HSFs cultured in vitro were randomly divided into a control group, UVB group, and anthocyanin groups (0.1, 0.5, and 1.0 mg/mL). HSFs in the UVB and anthocyanin groups were exposed to 30 mJ/cm2 UVB to establish a photoaging model. Then, apoptosis rate, tumor necrosis factor-α (TNF-α), cysteinyl aspartate specific proteinase-3 (caspase-3), cysteinyl aspartate specific proteinase-7 (caspase-7), and survivin expression were evaluated.

Results: UVB irradiation can increase the apoptosis rate of HSFs and expression of TNF-α, caspase-7, and survivin. Anthocyanin pretreatment (0.1, 0.5, and 1.0 mg/mL) decreased UVB-induced apoptosis rate and TNF-α and caspase-7 expression and increased survivin expression. Compared with the control group, the apoptosis rate and expression of TNF-α, caspase-7, and survivin of anthocyanin groups in UVB-irradiated HSFs were high. Among the three doses of anthocyanin (0.1, 0.5, and 1.0 mg/mL) groups, the apoptosis rate and TNF-α expression of anthocyanin at 1.0 mg/mL were the lowest. There was no significant change in caspase-3 expression in each group.

Conclusion: Anthocyanin from Lycium ruthenicum Murr. in the Qaidam Basin could alleviate UVB-induced apoptosis by regulating the death receptor pathway.

Keywords: Lycium ruthenicum Murr.; anthocyanin; apoptosis; human skin fibroblasts; ultraviolet B.

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Conflict of interest statement

All authors declare that they have no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Effect of anthocyanin on TNF-α in ultraviolet B (UVB)-induced human skin fibroblasts (HSFs). The expression of TNF-α was detected by ELISA. Data are shown as the mean ±SEM (n = 3). **p < 0.01 vs the control group. ##p < 0.01 vs the UVB group. ^^p < 0.01 vs the 1.0 mg/mL anthocyanin group.
Figure 2
Figure 2
Effect of anthocyanin on the expression of caspase-3, caspase-7, and survivin in ultraviolet B (UVB)-induced human skin fibroblasts (HSFs). Western blot was performed to determine the expression of caspase-3, caspase-7, and survivin. (A) Caspase-3, caspase-7, and survivin proteins in HSFs. (B) The expression of caspase-3, caspase-7, and survivin in HSFs. Data are shown as the mean ±SEM (n = 3).
Figure 3
Figure 3
Effect of anthocyanin on apoptosis rate in human skin fibroblasts (HSFs). The apoptosis rate was detected by flow cytometry. (A) Apoptosis rate in HSFs. (B) Comparison of apoptosis rate in HSFs. Data are shown as the mean ±SEM (n = 3). *p < 0.05,**p < 0.01 vs the control group. #p < 0.05, ##p < 0.01 vs the UVB group. ^^p < 0.01 vs the 1.0 mg/mL anthocyanin group.
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