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. 2023 Mar 17;4(1):101985.
doi: 10.1016/j.xpro.2022.101985. Epub 2023 Jan 3.

Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise

Stephen P Ashcroft  1 , Sara L Jepsen  2 , Amy M Ehrlich  1 , Jonas T Treebak  1 , Jens J Holst  3 , Juleen R Zierath  4
Affiliations

Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise

Stephen P Ashcroft et al. STAR Protoc. .

Abstract

The tissue-specific release and uptake of metabolites in response to exercise is incompletely understood. Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of mice and the region-specific sampling of blood from the liver and hindlimb. This procedure is particularly relevant for the study of tissue-specific metabolism in response to exercise. For complete details on the use and execution of this protocol, please refer to Sato et al. (2022).1.

Keywords: Behavior; Health Sciences; Metabolism; Model Organisms.

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Conflict of interest statement

Declaration of interests The authors declare no competing interests.

Figures

None
Graphical abstract
Figure 1
Figure 1
Hepatic blood sampling from the portal vein (A–F) and caudal vena cava (G–H)
Figure 2
Figure 2
Hindlimb blood sampling from the right common iliac vein (A–E) and abdominal aorta (F–G)
See this image and copyright information in PMC

References

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