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. 2023 Feb 15:302:120395.
doi: 10.1016/j.carbpol.2022.120395. Epub 2022 Nov 30.

3D bioprinted colorectal cancer models based on hyaluronic acid and signalling glycans

Francesca Cadamuro  1 , Laura Marongiu  2 , Michele Marino  3 , Nicolò Tamini  4 , Luca Nespoli  5 , Nicola Zucchini  6 , Alberta Terzi  7 , Davide Altamura  8 , Zirui Gao  9 , Cinzia Giannini  10 , Greta Bindi  11 , Andrew Smith  12 , Fulvio Magni  13 , Sabrina Bertini  14 , Francesca Granucci  15 , Francesco Nicotra  16 , Laura Russo  17
Affiliations
Free article

3D bioprinted colorectal cancer models based on hyaluronic acid and signalling glycans

Francesca Cadamuro et al. Carbohydr Polym. .
Free article

Abstract

In cancer microenvironment, aberrant glycosylation events of ECM proteins and cell surface receptors occur. We developed a protocol to generate 3D bioprinted models of colorectal cancer (CRC) crosslinking hyaluronic acid and gelatin functionalized with three signalling glycans characterized in CRC, 3'-Sialylgalactose, 6'-Sialylgalactose and 2'-Fucosylgalactose. The crosslinking, performed exploiting azide functionalized gelatin and hyaluronic acid and 4arm-PEG-dibenzocyclooctyne, resulted in biocompatible hydrogels that were 3D bioprinted with commercial CRC cells HT-29 and patient derived CRC tumoroids. The glycosylated hydrogels showed good 3D printability, biocompatibility and stability over the time. SEM and synchrotron radiation SAXS/WAXS analysis revealed the influence of glycosylation in the construct morphology, whereas MALDI-MS imaging showed that protein profiles of tumoroid cells vary with glycosylation, indicating that sialylation and fucosylation of ECM proteins induce diverse alterations to the proteome of the tumoroid and surrounding cells.

Keywords: 3D bioprinting; Bioresponsive hydrogels; Colorectal cancer models; Glycosignature; Hyaluronic acid.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.