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. 2022 Dec 26;13(1):86.
doi: 10.3390/ani13010086.

Effects of 25(OH)VD3 on Growth Performance, Pork Quality and Calcium Deposit in Growing-Finishing Pigs

Zeyu Zhang  1 , Gang Zhang  1 , Jindang Cao  2 , Baoqin Qiu  2 , Xiaoyu Qin  2 , Jinbiao Zhao  1
Affiliations

Effects of 25(OH)VD3 on Growth Performance, Pork Quality and Calcium Deposit in Growing-Finishing Pigs

Zeyu Zhang et al. Animals (Basel). .

Abstract

The study was conducted to evaluate the effects of 25(OH)VD3 with different inclusion levels of 0, 25, 50 and 75 μg/kg in the diet on growth performance, nutrient digestibility, bone properties and pork quality in growing-finishing pigs. The results showed that the average daily gain (p < 0.05) and body weight (p < 0.10) of pigs showed a trend of increasing quadratically as inclusion levels of 25(OH)VD3 increased. Dietary supplementation of 50 μg/kg 25(OH)VD3 increased calcium digestibility compared with the 0 μg/kg group (p < 0.05), and calcium and phosphorus digestibility increased quadratically as inclusion levels of 25(OH)VD3 increased (p < 0.05). Dietary supplementation of 50 μg/kg 25(OH)VD3 increased concentrations of polyunsaturated fatty acids, and decreased contents of saturated and monounsaturated fatty acids in the longissimus dorsi of pigs (p < 0.05). The addition of 25, 50 and 75 μg/kg 25(OH)VD3 to the diet increased breaking strength and bone stiffness in the tibia compared with the 0 μg/kg group (p < 0.05). Dietary supplementation of 50 μg/kg 25(OH)VD3 improved the activities of superoxide dismutase (SOD) and catalase (CAT), and increased the messenger RNA (mRNA) expression of Cu/Zn SOD in the longissimus dorsi compared with the 0 μg/kg group (p < 0.05). Supplementing 50 μg/kg 25(OH)VD3 improved the mRNA expression of calcium-binding protein D9k (CaBP-D9k) and D28k (CaBP-D28K) in the liver compared with the 0 μg/kg 25(OH)D3 group (p < 0.05). In conclusion, a diet with an added dose of 50 μg/kg 25(OH)VD3 showed a greatest growth performance of growing-finishing pigs, and 25(OH)VD3 enhanced calcium deposition and antioxidant capacity in the longissimus dorsi, which may be associated with improved expression of calcium ion channel proteins.

Keywords: 25 hydroxyvitamin D3; antioxidant capacity; calcium deposit; growing-finishing pigs; growth performance; pork quality.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Effects of 50 µg/kg 25(OH)VD3 on inflammatory cytokines concentration in the liver of growing-finishing pigs. Note: Superscript letters within the bar chart indicate significant differences (p < 0.05), n = 6. 25(OH)VD3, 25-hydroxyvitamin D3.
Figure 2
Figure 2
Effects of 50 µg/kg 25(OH)VD3 on antioxidase activity in the liver of growing-finishing pigs. (A) Concentration of liver antioxidase activity. (B) mRNA expression of antioxidase genes. Note: Superscript letters within the bar chart indicate significant differences (p < 0.05), n = 6. 25(OH)VD3, 25-hydroxyvitamin D3.
Figure 3
Figure 3
Effects of 50 µg/kg 25(OH)VD3 on the mRNA expression of calcium ion channel proteins in the liver of finishing pigs. Note: Superscript letters within the bar chart indicate significant differences (p < 0.05), n = 6. 25(OH)VD3, 25-hydroxyvitamin D3.
See this image and copyright information in PMC

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