Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Dec 23;24(1):241.
doi: 10.3390/ijms24010241.

CRISPR/Cas-Based Approaches to Study Schizophrenia and Other Neurodevelopmental Disorders

Artemiy O Kurishev  1 , Dmitry S Karpov  1   2 , Nonna I Nadolinskaia  3 , Anna V Goncharenko  3 , Vera E Golimbet  1
Affiliations
Review

CRISPR/Cas-Based Approaches to Study Schizophrenia and Other Neurodevelopmental Disorders

Artemiy O Kurishev et al. Int J Mol Sci. .

Abstract

The study of diseases of the central nervous system (CNS) at the molecular level is challenging because of the complexity of neural circuits and the huge number of specialized cell types. Moreover, genomic association studies have revealed the complex genetic architecture of schizophrenia and other genetically determined mental disorders. Investigating such complex genetic architecture to decipher the molecular basis of CNS pathologies requires the use of high-throughput models such as cells and their derivatives. The time is coming for high-throughput genetic technologies based on CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat)/Cas systems to manipulate multiple genomic targets. CRISPR/Cas systems provide the desired complexity, versatility, and flexibility to create novel genetic tools capable of both altering the DNA sequence and affecting its function at higher levels of genetic information flow. CRISPR/Cas tools make it possible to find and investigate the intricate relationship between the genotype and phenotype of neuronal cells. The purpose of this review is to discuss innovative CRISPR-based approaches for studying the molecular mechanisms of CNS pathologies using cellular models.

Keywords: CRISPR/Cas system; epigenome editing; genome editing; neurodevelopmental disorders; schizophrenia.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Models were used to study epigenetics of neurodevelopmental disorders.
See this image and copyright information in PMC

References

    1. Garcia-Gutierrez M.S., Navarrete F., Sala F., Gasparyan A., Austrich-Olivares A., Manzanares J. Biomarkers in psychiatry: Concept, definition, types and relevance to the clinical reality. Front. Psychiatry. 2020;11:432. doi: 10.3389/fpsyt.2020.00432. - DOI - PMC - PubMed
    1. Fabbri C. Genetics in psychiatry: Methods, clinical applications and future perspectives. Psychiatry Clin. Neurosci. Rep. 2022;1:e6. doi: 10.1002/pcn5.6. - DOI - PMC - PubMed
    1. Fuller T., Reus V. Shared genetics of psychiatric disorders. F1000Research. 2019;8:F1000. doi: 10.12688/f1000research.18130.1. - DOI - PMC - PubMed
    1. Poletti M., Raballo A. Before schizophrenia: Schizophrenic vulnerability in developmental age and its detection. Clin. Neuropsychiatry. 2021;18:293–295. - PMC - PubMed
    1. Mertens J., Wang Q.W., Kim Y., Yu D.X., Pham S., Yang B., Zheng Y., Diffenderfer K.E., Zhang J., Soltani S., et al. Differential responses to lithium in hyperexcitable neurons from patients with bipolar disorder. Nature. 2015;527:95–99. doi: 10.1038/nature15526. - DOI - PMC - PubMed