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. 2023 Jan 6;37(11):e371103.
doi: 10.1590/acb371103. eCollection 2023.

Study of the upper pole after subtotal splenectomy in rats

Amanda Lessa Martins  1 , Anna Bárbara Scárdua Parreira  1 , Maria Luiza Font Juliá Grossi  1 , Raquel de Azevedo Benevides  2 , Luciene Lage da Motta  3 , Lucia Helena Sagrillo Pimassoni  4 , Andrea Saade Daher Borjaili  5 , Marcela Souza Lima Paulo  6 , Danilo Nagib Salomão Paulo  7
Affiliations

Study of the upper pole after subtotal splenectomy in rats

Amanda Lessa Martins et al. Acta Cir Bras. .

Abstract

Purpose: To evaluate macro/microscopic viability of the upper pole (UP) in rats after 80 days of subtotal splenectomy preserving the upper pole (SSPUP).

Methods: Twenty-five male Wistar rats were submitted to SSPUP. After 80 days, the rats were euthanized, and the remaining UP was evaluated macroscopically regarding appearance, color, consistency, length, width, thickness, and presence of fibrosis/necrosis; and microscopically regarding presence of red and white pulp, fibrosis/necrosis.

Results: Two rats died during surgery and were removed from the statistical analysis. There was statistically significant increase in length and width between the pre and postoperative in the experimental group, with no significant difference in thickness. In the manipulation group, the macroscopic appearance of the spleen was normal in pre and postoperative, with viability preserved. In the experimental group, two UP of the spleen were not found during the second surgery. Macroscopically, it was observed absence of fibrosis and necrosis in all cases. Microscopically, the white and red pulp were intact in both groups. Two spleens of rats in the manipulation group presented areas with fibrosis and necrosis focus, which were not enough to be considered inviable.

Conclusions: The UP of the spleen remained viable in 91.3% of the cases.

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Conflict of interest statement

Conflict of interest: Nothing to declare.

Figures

Figure 1
Figure 1. Median laparotomy for examinationof the abdominal cavity.
Figure 2
Figure 2. Schematic drawing of the surgeryperformed in this study.
Figure 3
Figure 3. Immediate result after the removal of the middle and lower portion of the spleen in rats ,with preservation of the UP.
Figure 4
Figure 4. Late result, 80 days after the removal of the middle and lower portion of the spleen, with preservation of UP.
See this image and copyright information in PMC

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