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Comparative Study
. 1987 Jul 15;245(2):423-8.
doi: 10.1042/bj2450423.

The separation of glutathione transferase subunits by using reverse-phase high-pressure liquid chromatography

A K Ostlund Farrants  1 , D J Meyer, B Coles, C Southan, A Aitken, P J Johnson, B Ketterer
Affiliations
Comparative Study

The separation of glutathione transferase subunits by using reverse-phase high-pressure liquid chromatography

A K Ostlund Farrants et al. Biochem J. .

Abstract

A simple method is described for the separation and quantification of the subunits of GSH transferases present in rat tissue extracts. This method, involving GSH-agarose affinity chromatography followed by reverse-phase h.p.l.c., is rapid and sufficiently sensitive to measure 5 micrograms of each subunit in a mixture. Examples are given of its application to extracts of rat kidney, adrenal, testicular interstitial cells and seminiferous tubules. The analysis of seminiferous tubules indicates that the technique may be of value for the identification of novel subunits. Preliminary separations of subunits from human GSH transferases are also described.

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References

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