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. 2023 Mar;9(2):974-981.
doi: 10.1002/vms3.1074. Epub 2023 Jan 12.

Effect of oestrus synchronisation through ovulation delay by vaccination against foot-and-mouth disease in Hanwoo (Bos taurus coreanae) cows

Daehyun Kim  1 , Woo-Sung Kwon  2 , Jaejung Ha  1 , Jaejo Kim  3 , Danil Kim  4 , Wonyou Lee  5 , Joonho Moon  6 , Junkoo Yi  1
Affiliations

Effect of oestrus synchronisation through ovulation delay by vaccination against foot-and-mouth disease in Hanwoo (Bos taurus coreanae) cows

Daehyun Kim et al. Vet Med Sci. 2023 Mar.

Abstract

Background: In Korean cattle, after foot-and-mouth disease (FMD) vaccination, anovulation increases, acute immune response is stimulated.

Objective: Here, we aimed to improve the fertility rate by ovulation delay caused by the foot-and-mouth disease vaccine.

Methods: 160 cows (control, FMD, FMD+Gn250 and FMD+Gn500 groups, with 40 cows each) were used. We analysed the ovulation delay, ovulation rate, conception rate and acute-phase immune responses.

Results: In the group vaccinated only with FMD, the average follicle size was maintained at 12 mm and ovulation was delayed. The ovulation rate of the FMD+Gn500 group (500 μg gonadotropin-releasing hormone (GnRH) injections 3 days after the FMD vaccination) was the highest at 81.8%. The ovulation rate of the FMD+Gn250 group (250 μg GnRH injections 3 days after FMD vaccination) was 54.5%, and that of the control group (not FMD vaccinated) was 53.3%. The conception rate was 52.5% (19/40) in the control group, 37.5% (15/40) in the FMD+Gn250 group, and 67.5% (27/40) in the FMD+Gn500 group. Analysis of acute-phase immune response revealed that the plasma contents of haptoglobin and serum amyloid A increased up to 7 days after vaccination against FMD in all the experimental groups, except the control group.

Conclusions: We concluded that ovulation delay can be employed to improve conception rate after FMD vaccination through a modified ovulation synchronisation method with GnRH.

Keywords: Hanwoo (Bos taurus coreanae) cow; conception rate; foot-and-mouth disease vaccine; gonadotropin-releasing hormone; ovulation delay.

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Conflict of interest statement

We confirm that this manuscript has not been published in whole or in part and is not being considered for publication elsewhere. There are no any conflict of interest for all authors.

Figures

FIGURE 1
FIGURE 1
Schematic diagram of the experimental design. The diagonal square is the time of FMD vaccination, grey square is the time of i.m. injection, and circle is the time of ovulation test confirmed through transrectal ultrasonography. Blood collection was performed on day –3, 0, 3, 7 and 11.
FIGURE 2
FIGURE 2
Change in ovulation rate and follicle size depending on the different treatments (n = 160). (a) Change in ovulation rate by treatment. The black line connected by ‘○’ represents the mean ovulation rate of the control group (NOT vaccinated). The black line connected by ‘□’ represents the mean ovulation rate of the FMD group (Only vaccinated). The black line connected by ‘△’ represents the mean ovulation rate of the FMD+Gn250 group (250 μg GnRH i.m. injection on day 0). The black line connected by ‘▽’ represents the mean ovulation rate of the FMD+Gn500 group (500 μg GnRH i.m. injection on day 0). (b) Change of follicle size by treatment. The black line connected by ‘○’ represents the mean follicle size of the control group (Not vaccinated). The black line connected by ‘□’ represents the mean follicle size of the FMD group (Only vaccinated). The black line connected by ‘△’ represents the mean follicle size of the FMD+Gn250 group (250 μg GnRH i.m. injection at day 0). The black line connected by ‘▽’ represents the mean follicle size of the FMD+Gn500 group (500 μg GnRH i.m. injection at day 0). FMD, FMD+Gn250 and FMD+Gn500 group was injected FMD vaccine at day –3 (except for the control group). The control, FMD+Gn250 and FMD+Gn500 groups were injected with 25 mg PGF2α on day 7, injected with 250 μg GnRH on day 9 and artificial insemination was conducted on day 10. Blood samples were collected on days –3, 0, 3, 7 and 11.
FIGURE 3
FIGURE 3
Plasma concentrations of haptoglobin and serum amyloid A depending on the different treatments (n = 160). (a) Plasma haptoglobin and (b) serum amyloid A levels. The black line connected by ‘○’ represents the mean ovulation rate of the control group (NOT vaccinated). The black line connected by ‘□’ represents the mean ovulation rate of the FMD group (Only vaccinated). The black line connected by ‘△’ represents the mean ovulation rate of the FMD+Gn250 group (250 μg GnRH i.m. injection on day 0). The black line connected by ‘▽’ represents the mean ovulation rate of the FMD+Gn500 group (500 μg GnRH i.m. injection on day 0). The FMD, FMD+Gn250 and FMD+Gn500 groups were injected with the FMD vaccine on day –3 (except for the control group). The control, FMD+Gn250 and FMD+Gn500 groups were injected with 25 mg of PGF2α on day 7, injected with 250 μg GnRH on day 9 and artificial insemination on day 10. Blood collection was performed on day –3, 0, 3, 7 and 11. ***Significance level p < 0.001.
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