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Comment
. 2023 Mar 15;207(6):796-798.
doi: 10.1164/rccm.202212-2214LE.

Reply to Wang and Zhou

Katharina Jandl  1   2 , Leigh M Marsh  1   3 , Ayse Ceren Mutgan  1   3 , Slaven Crnkovic  1   3 , Francesco Valzano  1 , Diana Zabini  1   3 , Julia Hoffmann  1 , Vasile Foris  1   4 , Elisabeth Gschwandtner  5 , Walter Klepetko  5 , Helmut Prosch  6 , Holger Flick  4 , Luka Brcic  7 , Izidor Kern  8 , Akos Heinemann  2 , Horst Olschewski  1   4 , Gabor Kovacs  1   4 , Grazyna Kwapiszewska  1   3   9
Affiliations
Comment

Reply to Wang and Zhou

Katharina Jandl et al. Am J Respir Crit Care Med. .
No abstract available

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Figures

Figure 1.
Figure 1.
Reduced natural killer T (NKT) (-like) cell numbers are connected to vascular and parenchymal fibrosis. (A) Cluster of differentiation (CD) 3+CD56+ NKT-like cell numbers in freshly isolated pulmonary arteries from patients with pulmonary hypertension associated with pulmonary fibrosis (PF-PH) or chronic obstructive pulmonary disease (COPD-PH) compared with healthy donor controls (*P ⩽ 0.05 as determined by nonparametric ANOVA followed by Kruskal-Wallis multiple comparisons test). (B) Vascular collagen deposition was determined by quantification of Masson trichrome stainings. Vessel was defined by outer media border (**P ⩽ 0.005 as determined by nonparametric ANOVA followed by Kruskal-Wallis multiple comparisons test). (C) Quantification of various NKT (-like) cell subsets in patients with ILD or COPD with unknown pulmonary hemodynamic status. Peripheral blood mononuclear cells (PBMCs) were isolated from freshly drawn whole blood by density gradient centrifugation. PBMCs were stained for cell surface markers, and the CD14-negative population of PBMCs was quantified (n = 5–6; **P ⩽ 0.005, *P ⩽ 0.05 as determined by nonparametric Mann-Whitney test). (A and B) Donors and patients with PF-PH were described previously (1). ILD = interstitial lung disease.
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References

    1. Jandl K, Marsh LM, Mutgan AC, Crnkovic S, Valzano F, Zabini D, et al. Impairment of the NKT-STAT1-CXCL9 axis contributes to vessel fibrosis in pulmonary hypertension caused by lung fibrosis. Am J Respir Crit Care Med . 2022;206:981–998. - PubMed
    1. Hoffmann J, Wilhelm J, Marsh LM, Ghanim B, Klepetko W, Kovacs G, et al. Distinct differences in gene expression patterns in pulmonary arteries of patients with chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis with pulmonary hypertension. Am J Respir Crit Care Med . 2014;190:98–111. - PubMed
    1. Romero-Olmedo AJ, Schulz AR, Huber M, Brehm CU, Chang HD, Chiarolla CM, et al. Deep phenotypical characterization of human CD3+ CD56+ T cells by mass cytometry. Eur J Immunol . 2021;51:672–681. - PubMed
    1. Damayanti T, Kikuchi T, Zaini J, Daito H, Kanehira M, Kohu K, et al. Serial OX40 engagement on CD4+ T cells and natural killer T cells causes allergic airway inflammation. Am J Respir Crit Care Med . 2010;181:688–698. - PubMed
    1. Sharma AK, LaPar DJ, Zhao Y, Li L, Lau CL, Kron IL, et al. Natural killer T cell-derived IL-17 mediates lung ischemia-reperfusion injury. Am J Respir Crit Care Med . 2011;183:1539–1549. - PMC - PubMed

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