Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Apr;164(4):684-687.e4.
doi: 10.1053/j.gastro.2023.01.004. Epub 2023 Jan 11.

Trypsin Activity in Secretagogue-induced Murine Pancreatitis Is Solely Elicited by Cathepsin B and Does Not Mediate Key Pathologic Responses

Andrea Geisz  1 , Thanh Tran  2 , Anna Orekhova  2 , Miklós Sahin-Tóth  3
Affiliations

Trypsin Activity in Secretagogue-induced Murine Pancreatitis Is Solely Elicited by Cathepsin B and Does Not Mediate Key Pathologic Responses

Andrea Geisz et al. Gastroenterology. 2023 Apr.
No abstract available

PubMed Disclaimer

Conflict of interest statement

Conflict of interest: No conflicts to declare.

Figures

Figure 1.
Figure 1.
Effect of trypsinogen mutations D23A,K24G and D22A,K24G, and cathepsin B deficiency on cerulein-induced intrapancreatic trypsin activity and acute pancreatitis in mice. The three mutant strains were analyzed in separate experiments with their own C57BL/6N controls. For clarity, all results were graphed together and the C57BL/6N data were pooled, with light gray (T7D23A,K24G), dark gray (T7D22A,K24G), and black (Ctsb-KO) symbols indicating the different experiments. A, Primary structure of the trypsinogen activation peptide in mouse cationic trypsinogen (T7). The position of mutations D23A,K24G and D22A,K24G are indicated. The arrow points to the activation cleavage site. B, Biochemical pathways of intrapancreatic trypsinogen activation in mouse models with mutated T7 trypsinogen and Ctsb deletion (Ctsb-KO) alleles. C, Cerulein-induced intrapancreatic trypsin activity in T7D23A,K24G, T7D22A,K24G, Ctsb-KO, and C57BL/6N mice. Red and blue symbols highlight the increased and diminished trypsin activities in the T7D22A,K24G and Ctsb-KO mice, respectively. Trypsin activity was determined 30 min after a single injection of saline or cerulein. D, E, F, G, H, I, Acute pancreatitis in T7D23A,K24G, T7D22A,K24G, Ctsb-KO, and C57BL/6N mice given 10 hourly injections of normal saline or cerulein. D, Plasma amylase activity. E, Pancreas weight. F, Representative hematoxylin-eosin stained pancreas sections. Scale bars correspond to 100 μm. G, H, I, Histology scoring of pancreas sections for edema (G), inflammatory cells (H), and necrosis (I). Individual values with mean ± SD are shown. The difference of means between groups was analyzed by one-way ANOVA and Tukey-Kramer post-hoc test. See Methods in the Supplementary Material for experimental details.
See this image and copyright information in PMC

Comment in

References

    1. Hegyi E et al. Dig Dis Sci 2017; 62:1692–1701 - PMC - PubMed
    1. Geisz A et al. Nat Commun 2018; 9:5033. - PMC - PubMed
    1. Jancsó Z et al. Gastroenterology 2020; 158:1083–1094 - PMC - PubMed
    1. Van Acker GJ et al. Am J Physiol Gastrointest Liver Physiol 2002; 283:G794–800 - PubMed
    1. Halangk W et al. J Clin Invest 2000; 106:773–781 - PMC - PubMed

Publication types