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. 2023 Jan 16;13(1):802.
doi: 10.1038/s41598-023-27396-8.

A functional bimodal mesoporous silica nanoparticle with redox/cellulase dual-responsive gatekeepers for controlled release of fungicide

Weilan Huang #  1 , Hua Pan #  1 , Zhongxuan Hu  1 , Meijing Wang  1 , Litao Wu  1 , Fang Zhang  2
Affiliations

A functional bimodal mesoporous silica nanoparticle with redox/cellulase dual-responsive gatekeepers for controlled release of fungicide

Weilan Huang et al. Sci Rep. .

Abstract

Integrating toxic fungicide into a functional stimuli-responsive nanosystem can effectively improve the fungus control specificity and reduce the effect on non-target organisms. We report here a redox and cellulase dual-responsive multifunctional nanoparticle based on bimodal mesoporous silica (BMMs) to deliver prochloraz (Pro) for the smart management of wilt disease (Pro-AC-SS-BMMs, known as P-ASB). The surface of the nanocarrier was modified with an aminosilane coupling agent, and Pro was encapsulated by physical adsorption using 2,2'-dithiodiacetic acid as a smart bridge and disulfide (SS) cross-linked aminocellulose (AC) as gatekeepers. P-ASB nanoparticles (NPs) had a spherical structure, and the size was 531.2 ± 4.9 nm. The loading rate of Pro was 28.5%, and the NPs possessed excellent redox/cellulase dual-responsive release characteristics in the presence of glutathione (GSH) and cellulase. The nanocarrier could effectively protect Pro against photodegradation and had better foliar wettability than the Pro technical. Fluorescence tracer results showed that the nanocarriers were taken up and activated by the mycelium. P-ASB NPs had better control efficacy against Rhizoctonia solani and had no significant toxicity to cells and bacteria. This study provides a new strategy for enhancing the environmental protection and promoting the development of green agriculture.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Schematic diagram for preparation of P-ASB nanoparticle and redox/cellulase dual-responsive release mechanism.
Figure 2
Figure 2
The mechanism for preparation of the P-ASB NPs.
Figure 3
Figure 3
TEM images of BMMs (A, B) and P-ASB NPs (C, D).
Figure 4
Figure 4
N2 adsorption–desorption isotherms (A) and pore size distribution (B) of P-ASB NPs and control samples.
Figure 5
Figure 5
Small-angle XRPD patterns (A), FTIR spectra (B), Raman spectrum and element contents (C) and TGA profiles (D) of P-ASB NPs and control samples.
Figure 6
Figure 6
Effects of pH value (A), GSH concentrations (B), cellulase (C) and cellulase + GSH (D) on the release behaviors of Pro from P-ASB NPs.
Figure 7
Figure 7
Photostability of P-ASB NPs under UV-light irradiation.
Figure 8
Figure 8
Contact angle images and values of P-ASB NPs and control samples on cucumber leaves.
Figure 9
Figure 9
Fluorescence images of R. solani incubated with ASB/FITC and blank control for 7 days. The laser excitation wavelength was 488 nm.
Figure 10
Figure 10
Fungicidal activity and inhibitory rates of P-ASB NPs in vitro against R. solani on the 12th day.
Figure 11
Figure 11
Biosafety evaluations of different concentrations of ASB nanocarriers.
See this image and copyright information in PMC

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