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. 2023 Mar-Apr;13(2):164-168.
doi: 10.1016/j.jobcr.2023.01.003. Epub 2023 Jan 6.

CaCO3 from Anadara granosa shell as reparative dentin inducer in odontoblast pulp cells: In-vivo study

Widya Saraswati  1 , Adioro Soetojo  1 , Nina Dhaniar  2 , Hermawan Adi Praja  2 , Ratih Mahanani Santoso  2 , Nadiah Salsabila Nosla  2 , Ganiezha Cindananti  2 , Rossabella Vennowusky Rafli  2 , Nabiela Rahardia  2
Affiliations

CaCO3 from Anadara granosa shell as reparative dentin inducer in odontoblast pulp cells: In-vivo study

Widya Saraswati et al. J Oral Biol Craniofac Res. 2023 Mar-Apr.

Erratum in

Abstract

Introduction: Anadara granosa (blood clam) shell contained 98.7% of calcium carbonate (CaCO3). This material has bio-properties that able to induced the dentin regeneration. This study is expected to reveal the nuclear factor kappa beta (NF-kB), transforming growth factor beta (TGF-β1), and vascular endothelial growth factor A (VEGF-A) expression in dental pulp after application of CaCO3 from Anadara granosa shell.

Material and methods: The thirty Rattus norvegicus strain Wistar used as model. The maxillary first molar was preparation using 0.84 mm low-speed diamond bur to made cavity. The cavity then applied glass ionomer cement (as control group) and other group applied CaCO3 from Anadara granosa shell. The teeth in each group were extracted after 1st, 3rd and 7th days of application for immunohistochemistry analysis for NF-kB, TGF-β1, and VEGF-A expression.

Result: The NF-kB expression in the group with CaCO3 from Anadara granosa shell lower than control after 1st, 3rd and 7th days (p < 0.05). In other hand, the TGF-β1 and VEGF-A expression in the group with CaCO3 from Anadara granosa shell higher than control after 1st, 3rd and 7th days (p < 0.05).

Conclusion: CaCO3 from Anadara granosa shell able to stimulate the TGF-β1 and VEGF-A and suppress the NF-kB expression in the dental pulp. This material able to develop as dentin-pulp material restoration.

Keywords: Anadara granosa; CaCO3; NF-kB; Reparative dentin; TGF-β1; VEGF-A.

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Conflict of interest statement

All author declares no conflict of interest

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
The NF-kB expression at 1000× magnification. Control group (A), CaCO3 from Anadara Granosa shell after 1st day (B), CaCO3 from Anadara Granosa shell after 3rd day (C) and CaCO3 from Anadara Granosa shell after 7th day (D).
Fig. 2
Fig. 2
The TGF-β expression at 1000× magnification. Control group (A), CaCO3 from Anadara Granosa shell after 1st day (B), CaCO3 from Anadara Granosa shell after 3rd day (C) and CaCO3 from Anadara Granosa shell after 7th day (D).
Fig. 3
Fig. 3
The VEGF-A expression at 1000× magnification. Control group (A), CaCO3 from Anadara Granosa shell after 1st day (B), CaCO3 from Anadara Granosa shell after 3rd day (C) and CaCO3 from Anadara Granosa shell after 7th day (D).
See this image and copyright information in PMC

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