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. 2023 Aug;68(4):571-577.
doi: 10.1007/s12223-023-01034-2. Epub 2023 Jan 19.

Examining the effects of BRG1 over-expression on Candida albicans strains growing as pseudohyphae

Joseph Mariscal  1 , Derek P Thomas  1 , Ian A Cleary  2
Affiliations

Examining the effects of BRG1 over-expression on Candida albicans strains growing as pseudohyphae

Joseph Mariscal et al. Folia Microbiol (Praha). 2023 Aug.

Abstract

The pathogen Candida albicans is pleiomorphic and grows in yeast and filamentous forms but the relationship between the regulation of different filamentous forms is unclear. BRG1 encodes a DNA binding protein which is an important regulator of morphology. Mutants lacking BRG1 grow as yeast under all conditions tested and over-expressing BRG1 drives hyphal growth even in the absence of inducing signals. A number of genetic mutants in repressors of filamentation form pseudohyphae under yeast conditions and some of these mutants can form hyphae under hypha-inducing conditions. This study examines the position of BRG1 in the regulatory networks that govern filamentation by examining the effect of over-expressing BRG1 in pseudohyphal mutants.

Keywords: BRG1; Candida albicans; NRG1; RFG1; TUP1 pseudohyphae.

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References

    1. Bensen ES, Filler SG, Berman J (2002) A forkhead transcription factor is important for true hyphal as well as yeast morphogenesis in Candida albicans. Eukaryot Cell 1:787–798. https://doi.org/10.1128/EC.1.5.787-798.2002 - DOI - PubMed - PMC
    1. Braun BR, Johnson AD (1997) Control of filament formation in Candida albicans by the transcriptional repressor TUP1. Science 277:105–109. https://doi.org/10.1126/science.277.5322.105 - DOI - PubMed
    1. Braun BR, Kadosh D, Johnson AD (2001) NRG1, a repressor of filamentous growth in C.albicans, is down-regulated during filament induction. EMBO J 20:4753–4761. https://doi.org/10.1093/emboj/20.17.4753 - DOI - PubMed - PMC
    1. Care RS, Trevethick J, Binley KM, Sudbery PE (1999) The MET3 promoter: a new tool for Candida albicans molecular genetics. Mol Microbiol 34:792–798. https://doi.org/10.1046/j.1365-2958.1999.01641.x - DOI - PubMed
    1. Cleary IA, Lazzell AL, Monteagudo C et al (2012) BRG1 and NRG1 form a novel feedback circuit regulating Candida albicans hypha formation and virulence. Mol Microbiol 85:557–573. https://doi.org/10.1111/j.1365-2958.2012.08127.x - DOI - PubMed - PMC

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