Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023:2619:53-60.
doi: 10.1007/978-1-0716-2946-8_4.

Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular Matrix

Arianna Parnigoni  1 , Paola Moretto  1 , Simona Rovera  1 , Manuela Viola  1 , Evgenia Karousou  1 , Alberto Passi  1 , Davide Vigetti  2
Affiliations

Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular Matrix

Arianna Parnigoni et al. Methods Mol Biol. 2023.

Abstract

Hyaluronan (HA) is the most abundant glycosaminoglycan in the extracellular matrix, and its deposition is strictly related to changes in cellular behaviors, such as cell migration, proliferation, and adhesion. Pericellular HA is abundant in a variety of cell types, and its amount could reflect specific conditions, thus suggesting a particular cellular status.Particle exclusion assay is a useful tool to visualize pericellular matrices with a high HA content, simply employing microscope image analysis. This approach is quick and allows to visualize the presence of a clear pericellular region around single cells, where fixed red blood cells are excluded if the pericellular matrix has been deposited.

Keywords: Extracellular matrix; Glycosaminoglycan; Hyaluronan; Particle exclusion; Pericellular matrix.

PubMed Disclaimer

References

    1. Karamanos NK, Piperigkou Z, Theocharis AD et al (2018) Proteoglycan chemical diversity drives multifunctional cell regulation and therapeutics. Chem Rev 118:9152–9232. https://doi.org/10.1021/acs.chemrev.8b00354
    1. Heldin P, Basu K, Olofsson B et al (2013) Deregulation of hyaluronan synthesis, degradation and binding promotes breast cancer. J Biochem 154:395–408 - DOI
    1. Udabage L, Brownlee GR, Waltham M et al (2005) Antisense-mediated suppression of hyaluronan synthase 2 inhibits the tumorigenesis and progression of breast cancer. Cancer Res 65:6139–6150. https://doi.org/10.1158/0008-5472.CAN-04-1622 - DOI
    1. Caon I, Parnigoni A, Viola M et al (2021) Cell energy metabolism and hyaluronan synthesis. J Histochem Cytochem 69:35–47. https://doi.org/10.1369/0022155420929772 - DOI
    1. Viola M, Karousou E, D’Angelo L et al (2016) Extracellular matrix in atherosclerosis: hyaluronan and proteoglycans insights. Curr Med Chem 23:2958–2971. https://doi.org/10.2174/0929867323666160607104602 - DOI

MeSH terms

LinkOut - more resources