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. 2023:2619:169-186.
doi: 10.1007/978-1-0716-2946-8_13.

In Vitro Investigation of Hyaluronan/CD44 Network

Theodore Karalis  1   2 , Paraskevi Heldin  2 , Spyros S Skandalis  3
Affiliations

In Vitro Investigation of Hyaluronan/CD44 Network

Theodore Karalis et al. Methods Mol Biol. 2023.

Abstract

Hyaluronan is one of the most influential components of the extracellular matrix. It is involved in the regulation of normal tissue function and architecture, while its metabolism is perturbed in a multitude of human diseases like inflammation, cancer, and viral infection. Given the implication of hyaluronan in a vast array of diseases, we describe here assays that can be utilized to study the quantity, size, subcellular localization, and binding capacity of hyaluronan by cells as well as its interactions with its major cellular receptor, CD44. Hopefully, these protocols will provide researchers with useful tools to study the complex hyaluronan biology.

Keywords: 4-MU; CD44; Cell adhesion; HAS2; Hyaluronan; Hyaluronan/CD44 interactions.

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References

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    1. Karalis T, Skandalis SS (2022) Hyaluronan network: a driving force in cancer progression. Am J Physiol Cell Physiol 323:C145 - DOI
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    1. Skandalis SS, Karalis TT, Chatzopoulos A et al (2019) Hyaluronan-CD44 axis orchestrates cancer stem cell functions. Cell Signal 63:109377 - DOI

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