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. 2022 Dec 26;11(1):56.
doi: 10.3390/biomedicines11010056.

The Role of LINC01564, RAMS11, CBX4 and TOP2A in Hepatocellular Carcinoma

Eman A E Badr  1 , Elshaymaa I Elmongy  2 , Rasha Galal Mostafa  3 , Ibrahim El-Tantawy El-Sayed  4 , Abd El-Naser Abd El-Ati Gad Allah  5 , Asmaa Khairy Ahmed  4 , Yasser A S Elghobashy  1
Affiliations

The Role of LINC01564, RAMS11, CBX4 and TOP2A in Hepatocellular Carcinoma

Eman A E Badr et al. Biomedicines. .

Abstract

Background: Hepatocellular carcinoma (HCC) is the most common histologic type of primary liver cancers worldwide. Hepatitis C virus (HCV) infection remains a major risk factor for chronic liver disease, cirrhosis, and HCC. To understand the molecular pathogenesis of HCC in chronic HCV infection, many molecular markers are extensively studied, including long noncoding RNAs (lncRNA). Objective: To evaluate the expression levels of lncRNAs (LINC01564, RAMS11), CBX4, and TOP2A in patients with chronic HCV infection and patients with HCC on top of chronic HCV infection and correlate these levels with the clinicopathological features of HCC. Subjects and Methods: One hundred and fifty subjects were enrolled in this study and divided into three groups: group I included 50 patients with HCC on top of chronic hepatitis C (CHC), group II included 50 patients with CHC only, and group III included 50 healthy individuals as a control group. LncRNAs relative expression level was determined by RT-PCR. Results: lncRNA (LINC01564, RAMS11), CBX4, and TOP2A relative expression levels were upregulated in both patient groups compared to controls (p < 0.001*), with the highest levels in the HCC group compared with the CHC group. Additionally, these levels were significantly positively correlated with the clinicopathological features of HCC. Conclusions: The lncRNA (LINC01564, RAMS11), CBX4, and TOP2A relative expression levels were upregulated in CHC patients—in particular, patients with HCC. Thus, these circulatory lncRNAs may be able to serve as promising noninvasive diagnostic markers for HCC associated with viral C hepatitis.

Keywords: CBX4; HCC; LINC01564; RAMS11 and TOP2A; chronic hepatitis C; lncRNA.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
Amplification plot for the first run.
Figure 2
Figure 2
Melting curve for the first run.
Figure 3
Figure 3
ROC curve for different parameters to discriminate HCC group form HCV group.
See this image and copyright information in PMC

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