Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Dec 30;9(1):59.
doi: 10.3390/jof9010059.

Molecular Diagnosis of Endemic Mycoses

Clara Valero  1 , María Teresa Martín-Gómez  2 , María José Buitrago  3   4
Affiliations
Review

Molecular Diagnosis of Endemic Mycoses

Clara Valero et al. J Fungi (Basel). .

Abstract

Diagnosis of endemic mycoses is still challenging. The moderated availability of reliable diagnostic methods, the lack of clinical suspicion out of endemic areas and the limitations of conventional techniques result in a late diagnosis that, in turn, delays the implementation of the correct antifungal therapy. In recent years, molecular methods have emerged as promising tools for the rapid diagnosis of endemic mycoses. However, the absence of a consensus among laboratories and the reduced availability of commercial tests compromises the diagnostic effectiveness of these methods. In this review, we summarize the advantages and limitations of molecular methods for the diagnosis of endemic mycoses.

Keywords: NGS; PCR; endemic mycoses; molecular diagnosis.

PubMed Disclaimer

Conflict of interest statement

M.J.B. is a founding partner and holds shares of Micologia Molecular S.L. She has received grant support from the Instituto de Salud Carlos III and has been paid for talks on behalf of United Medical LTDA.

Figures

Figure 1
Figure 1
Description of the technical characteristics, advantages and limitations of the molecular methods used for the diagnosis of endemic mycoses including specific PCR methods, methods based on broad range PCR and new methods as those based on Next Generation Sequencing (NGS). MCA: melting curve analysis.
See this image and copyright information in PMC

References

    1. Gnat S., Lagowski D., Nowakiewicz A., Dylag M. A global view on fungal infections in humans and animals: Infections caused by dimorphic fungi and dermatophytoses. J. Appl. Microbiol. 2021;131:2688–2704. doi: 10.1111/jam.15084. - DOI - PubMed
    1. WHO . Fungal Priority Pathogens List to Guide Research, Development and Public Health Action. World Health Organization; Geneva, Switzerland: 2022.
    1. Shikanai-Yasuda M.A., Mendes R.P., Colombo A.L., Queiroz-Telles F., Kono A.S.G., Paniago A.M.M., Nathan A., Valle A., Bagagli E., Benard G., et al. Brazilian guidelines for the clinical management of paracoccidioidomycosis. Rev. Soc. Bras. Med. Trop. 2017;50:715–740. doi: 10.1590/0037-8682-0230-2017. - DOI - PubMed
    1. Thompson G.R., 3rd, Le T., Chindamporn A., Kauffman C.A., Alastruey-Izquierdo A., Ampel N.M., Andes D.R., Armstrong-James D., Ayanlowo O., Baddley J.W., et al. Global guideline for the diagnosis and management of the endemic mycoses: An initiative of the European Confederation of Medical Mycology in cooperation with the International Society for Human and Animal Mycology. Lancet Infect. Dis. 2021;21:e364–e374. doi: 10.1016/S1473-3099(21)00191-2. - DOI - PMC - PubMed
    1. Gorris M.E., Cat L.A., Zender C.S., Treseder K.K., Randerson J.T. Coccidioidomycosis Dynamics in Relation to Climate in the Southwestern United States. Geohealth. 2018;2:6–24. doi: 10.1002/2017GH000095. - DOI - PMC - PubMed

LinkOut - more resources