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. 2023 Jan 5;13(2):236.
doi: 10.3390/nano13020236.

Biocompatibility and Antimicrobial Activity of Electrospun Fibrous Materials Based on PHB and Modified with Hemin

Polina M Tyubaeva  1   2 , Ivetta A Varyan  1   2 , Elena D Nikolskaya  1 , Mariia R Mollaeva  1 , Nikita G Yabbarov  1 , Maria B Sokol  1 , Margarita V Chirkina  1 , Anatoly A Popov  1   2
Affiliations

Biocompatibility and Antimicrobial Activity of Electrospun Fibrous Materials Based on PHB and Modified with Hemin

Polina M Tyubaeva et al. Nanomaterials (Basel). .

Abstract

The effect of the hemin (Hmi) on the structure and properties of nanocomposite electrospun materials based on poly-3-hydroxybutyrate (PHB) is discussed in the article. The additive significantly affected the morphology of fibers allowed to produce more elastic material and provided high antimicrobial activity. The article considers also the impact of the hemin on the biocompatibility of the nonwoven material based on PHB and the prospects for wound healing.

Keywords: antimicrobial activity; biocompatibility; electrospun fibrous materials; hemin; poly-3-hydroxybutyrate.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Structural formulas of PHB (a) and hemin (b).
Figure 2
Figure 2
SEM images of electrospun materials based on PHB with different content of Hmi: 0% wt. (a), 1% wt. (b), 3% wt., (c) and 5% wt. (d).
Figure 3
Figure 3
Typical tensile stress-strain curves of electrospun materials based on PHB with different content of Hmi: 0% wt. (blue), 1% wt. (yellow), 3% wt., (grey) and 5% wt. (red).
Figure 4
Figure 4
Degree of crystallinity (a) and average sizes of PHB crystallites L020 (b) of PHB–Hmi composites.
Figure 5
Figure 5
Contact wetting angles of the fibrous materials based on PHB–Hmi.
Figure 6
Figure 6
Air volume that passed through the fibrous materials based on PHB–Hmi according to the Gurley method.
Figure 7
Figure 7
The changes in the Hmi concentration in PBS solution (pH 7.4, 37 °C).
Figure 8
Figure 8
The optical microscopic images of PHB with different hemin content: 0% wt. (a), 1% wt. (b), 3% wt. (c), and 5% wt. (d) before fibroblasts seeding BJ-5ta cells. Bar—50 µm.
Figure 9
Figure 9
The optical microscopic images of the fibroblasts BJ-5ta after 24, 48 and 72 h (the images aligned left to right respectively) of cultivation in presence of PHB with different content of the hemin: 0% wt. (b), 1% wt. (c), 3% wt. (d), and 5% wt. (e); (a) control. Bar—50 µm.
Figure 9
Figure 9
The optical microscopic images of the fibroblasts BJ-5ta after 24, 48 and 72 h (the images aligned left to right respectively) of cultivation in presence of PHB with different content of the hemin: 0% wt. (b), 1% wt. (c), 3% wt. (d), and 5% wt. (e); (a) control. Bar—50 µm.
Figure 10
Figure 10
Survival of BJ-5ta cells after 72 h incubation with the PHB with different content of the hemin (0—0% wt., 1—1% wt., 3—3% wt., and 5—5% wt). (mean ± SD, n = 3).
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