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. 2022 Dec 23;16(1):21.
doi: 10.3390/ph16010021.

Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts

Nikoleta Đorđevski  1 , Abdullahi Ibrahim Uba  2 , Gokhan Zengin  3 , Jelena Božunović  4 , Uroš Gašić  4 , Elizabeta Ristanović  1 , Ana Ćirić  4 , Biljana Nikolić  5 , Dejan Stojković  4
Affiliations

Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts

Nikoleta Đorđevski et al. Pharmaceuticals (Basel). .

Abstract

This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of Allium scorodoprasum L. (Amaryllidaceae)-the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(-)HESI-QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3-O-glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of A. scorodoprasum with contemporary in vitro scientific approaches.

Keywords: Alllium scorodoprasum L. flowers; anti-enzymatic; antimicrobial; antioxidant; clinical isolates; medieval remedy; molecular modeling; wound healing.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Relative growth rate of HaCaT cells in the presence of A. scorodoprasum flower extracts at concentration 400 μg/mL.
Figure 2
Figure 2
Binding energy (docking) scores of the bioactive compounds from the tested extracts against each of the target enzymes.
Figure 3
Figure 3
Protein–ligand interactions: (A) AChE and rutin, (B) BChE and kaempferol 3-O-glucoside, (C) tyrosinase and rutin, (D) amylase and kaempferol and (E) glucosidase and 5-O-caffeoylquinic acid.
See this image and copyright information in PMC

References

    1. Jarić S., Mitrović M., Djurdjević L., Kostić O., Gajić G., Pavlović D., Pavlović P. Phytotherapy in medieval Serbian medicine according to the pharmacological manuscripts of the Chilandar Medical Codex (15-16th centuries) J. Ethnopharmacol. 2011;137:601–619. doi: 10.1016/j.jep.2011.06.016. - DOI - PubMed
    1. Dusanka P., Dragan S. The Chilander Medical Codex: Its importance for the medieval Serbian pharmacy. Pharm. Hist. Aust. 2001;14:12854602. - PubMed
    1. Djordjević S.P. Hilandarski medicinski kodeks. Srp. Arh. Celok. Lek. 1980;108:961–967. - PubMed
    1. Barile E., Capasso R., Izzo A.A., Lanzotti V., Sajjadi S.E., Zolfaghari B. Structure-activity relationships for saponins from Allium hirtifolium and Allium elburzense and their antispasmodic activity. Planta Med. 2005;71:1010–1018. doi: 10.1055/s-2005-873134. - DOI - PubMed
    1. Elgorashi E.E., Van Staden J. Bioactivity and bioactive compounds of African amaryllidaceae. ACS Symp. Ser. 2009;1021:151–170. doi: 10.1021/BK-2009-1021.CH008. - DOI

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