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. 2023 Jan 12;12(2):358.
doi: 10.3390/plants12020358.

Study of the Chemical Profile and Anti-Fungal Activity against Candida auris of Cinnamomum cassia Essential Oil and of Its Nano-Formulations Based on Polycaprolactone

Roberto Rosato  1 , Edoardo Napoli  2 , Giuseppe Granata  2 , Maura Di Vito  1 , Stefania Garzoli  3 , Corrada Geraci  2 , Silvia Rizzo  1 , Riccardo Torelli  4 , Maurizio Sanguinetti  1   4 , Francesca Bugli  1   4
Affiliations

Study of the Chemical Profile and Anti-Fungal Activity against Candida auris of Cinnamomum cassia Essential Oil and of Its Nano-Formulations Based on Polycaprolactone

Roberto Rosato et al. Plants (Basel). .

Abstract

Background: Candida auris represents an emerging pathogen that results in nosocomial infections and is considered a serious global health problem. The aim of this work was to evaluate the in vitro antifungal efficacy of Cinnamomum cassia essential oil (CC-EO) pure or formulated in polycaprolactone (PCL) nanoparticles against ten clinical strains of C. auris.

Methods: nanoparticles of PCL were produced using CC-EO (nano-CC-EO) and cinnamaldehyde (CIN) through the nanoprecipitation method. The chemical profile of both CC-EO and nano-CC-EO was evaluated using SPME sampling followed by GC-MS analysis. Micro-broth dilution tests were performed to evaluate both fungistatic and fungicidal effectiveness of CC-EO and CIN, pure and nano-formulated. Furthermore, checkerboard tests to evaluate the synergistic action of CC-EO or nano-CC-EO with micafungin or fluconazole were conducted. Finally, the biofilm disrupting activity of both formulations was evaluated.

Results: GC-MS analysis shows a different composition between CC-EO and nano-CC-EO. Moreover, the microbiological analyses do not show any variation in antifungal effectiveness either towards the planktonic form (MICCC-EO = 0.01 ± 0.01 and MICnano-CC-EO = 0.02 ± 0.01) or the biofilm form. No synergistic activity with the antifungal drugs tested was found.

Conclusions: both CC-EO and nano-CC-EO show the same antimicrobial effectiveness and are potential assets in the fight against C. auris.

Keywords: antifungal properties; cinnamaldehyde; cinnamon.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Eradication of performed biofilm. (**) indicate a statistical significance corresponding to p < 0.001.
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