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Comment
. 2022 Dec 29;15(1):89.
doi: 10.3390/v15010089.

Should SVGp12 Be Used for JC Polyomavirus Studies? Comment on Prezioso et al. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains. Viruses 2022, 14, 2070

Stian Henriksen  1   2 , Christine Hanssen Rinaldo  1   2
Affiliations
Comment

Should SVGp12 Be Used for JC Polyomavirus Studies? Comment on Prezioso et al. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains. Viruses 2022, 14, 2070

Stian Henriksen et al. Viruses. .

Abstract

A recent paper in Viruses investigates the impact of the JC polyomavirus (JCPyV) microRNA on the replication of different JCPyV strains. Unfortunately, one of the cell lines used, the human fetal glial cell line SVGp12, is productively infected by the closely related BK polyomavirus (BKPyV), which may confound results. Scientists need to take this into account and the potential pitfalls.

Keywords: BKPyV; JCPyV; SVGp12; exosomes; infection; microRNA; polyomavirus; replication.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The SVGp12 cell line expresses BKPyV proteins and can be infected by JCPyV. SVGp12 cells were (A) mock-infected or (B) infected with JCPyV (Mad-4). Cells were fixed 4 d.p.i. and indirect immunofluorescence staining was performed using a BKPyV-specific agnoprotein rabbit polyclonal antiserum in combination with a JCPyV-specific Vp1 mouse monoclonal antibody (ab34756; Abcam). As secondary antibodies goat-anti-rabbit (green, Alexa 488) and goat-anti-mouse (red, Alexa Fluor 568) were used. The DNA (nucleus) was stained with Draq5 (blue). Both images were acquired using a Nikon light microscope with a 10× and 20× objective, respectively.
See this image and copyright information in PMC

Comment on

References

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