Efficient lead immobilization by bio-beads containing Pseudomonas rhodesiae and bone char

Abstract

Mineralization of lead ions (Pb²⁺) to pyromorphite using phosphorus-containing materials is an effective way to remediate lead (Pb) contamination. Bone char is rich in phosphorus, but its immobilization of Pb²⁺ is limited by poor phosphate release. To utilize the phosphorus in bone char and provide a suitable growth environment for phosphate-solubilizing bacteria, bone char and Pseudomonas rhodesiae HP-7 were encapsulated into bio-beads, and the immobilization performance and mechanism of Pb in solution and soil by bio-beads were investigated. The results showed that 137 mg/g of phosphorus was released from bone char in the presence of the HP-7 strain. Pb²⁺ removal efficiency reached 100 % with an initial Pb²⁺ concentration of 1 mM, bone char content of 6 g/L, and bio-bead dosage of 1 %. Most Pb²⁺ was immobilized on the surface of the bio-beads as Pb₅(PO₄)₃Cl. The soil remediation experiments showed a 34 % reduction in the acid-soluble fraction of Pb. The bio-beads showed good stability in long-term (30 d) soil remediation. The present study shows that bone char can be turned into an efficient Pb immobilization material in the presence of phosphate-solubilizing bacteria. Thus, bio-beads are expected to be used in the remediation of Pb-contaminated environments.

Keywords: Bioremediation; Bone char; Encapsulation; Lead; Phosphate-solubilizing bacteria.