Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Mar;102(3):102483.
doi: 10.1016/j.psj.2023.102483. Epub 2023 Jan 7.

Drug resistance analysis of three types of avian-origin carbapenem-resistant Enterobacteriaceae in Shandong Province, China

Yaxin Su  1 , Li Xin  2 , Feng Zhang  3 , Chong Peng  1 , Zixuan Li  1 , Cong Liu  1 , Fangkun Wang  4
Affiliations

Drug resistance analysis of three types of avian-origin carbapenem-resistant Enterobacteriaceae in Shandong Province, China

Yaxin Su et al. Poult Sci. 2023 Mar.

Abstract

Animal-derived Enterobacteriaceae bacteria such as Escherichia coli (E. coli), Proteus mirabilis (P. mirabilis), and Klebsiella pneumoniae (K. pneumoniae) are important food-borne zoonotic bacilli that exist widely in the broiler-breeding industry. Although carbapenem antibiotics are considered to be the last line of defense against multidrug-resistant bacteria, carbapenem-resistant Enterobacteriaceae (CRE) break through them. In our study, we therefore, examined the prevalence of CRE and characteristics of antimicrobial resistance in 6 conventional broiler-fattening farms in Shandong Province, China. Our study revealed isolation rates of 3.57% (6/168) for carbapenem-resistant E. coli, 10% (5/50) for carbapenem-resistant P. mirabilis, and 3.03% (1/33) for carbapenem-resistant K. pneumoniae. All 12 CRE bacterial strains showed varying degrees of resistance to 27 antibiotics in 8 classes and were multidrug-resistant. The rate of the strains containing blaNDM genes, at 91.67% (11/12), was especially high. Among other results, the carrying rate of integrons in CRE bacteria was 91.67% (11/12), and 2 strains carried both class I and class II integrons, which accelerated the lateral transmission of resistant bacteria. Our first-ever finding of the 3 CRE bacteria E. coli, P. mirabilis, and K. pneumoniae on the same broiler farm suggests that poultry-derived CRE strains may pose a risk to humans. Moreover, our findings from surveillance can inform current understandings of the prevalence and characteristics of multidrug-resistant CRE in Shandong Province and, in turn, help to curb threats to food safety and public health and better prevent and control infectious zoonotic diseases.

Keywords: bla(NDM); carbapenem; drug resistance gene; multidrug resistance.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Geographic distribution of the sampling sites and CRE isolates across different prefecture-level cities in Shandong, China. A total of 12 CRE bacteria samples were collected from 6 broiler fattening farms in the Tai'an, Linyi, Liaocheng, Weifang, Binzhou, and Heze areas. Among them, Weifang and Binzhou have no CRE bacteria.
Figure 2
Figure 2
Drug resistance status of CRE bacteria. (A) Resistance rate of CRE bacteria to some antimicrobial agents. (B) The multidrug resistance of CRE bacteria. (C) CRE bacteria resistance spectrum, red indicates resistance, green indicates sensitivity, yellow indicates moderate sensitivity.
Figure 3
Figure 3
Drug resistance genes of CRE. (A) The detection rates of antibiotic resistance genes in CRE isolates. (B) Detection rates of diverse classes of ARGs in CRE isolates.
See this image and copyright information in PMC

References

    1. Aires-de-Sousa M., Ortiz de la Rosa J.M., Gonçalves M.L., Pereira A.L., Nordmann P., Poirel L. Epidemiology of carbapenemase-producing Klebsiella pneumoniae in a hospital, Portugal. Emerg. Infect. Dis. 2019;25:1632–1638. - PMC - PubMed
    1. Algammal A.M., Hashem H.R., Alfifi K.J., Hetta H.F., Sheraba N.S., Ramadan H., El-Tarabili R.M. atpD gene sequencing, multidrug resistance traits, virulence-determinants, and antimicrobial resistance genes of emerging XDR and MDR-Proteus mirabilis. Sci. Rep. 2021;11:9476. - PMC - PubMed
    1. Bi S., Yan H., Chen M., Zhang Z., Shi L., Wang H. New variant Salmonella genomic island 1-U in Proteus mirabilis clinical and food isolates from South China. J. Antimicrob. Chemother. 2011;66:1178–1179. - PubMed
    1. Bocharova Y., Savinova T., Lazareva A., Polikarpova S., Gordinskaya N., Mayanskiy N., Chebotar I. Genotypes, carbapenemase carriage, integron diversity and oprD alterations among carbapenem-resistant Pseudomonas aeruginosa from Russia. Int. J. Antimicrob. Agents. 2020;55 - PubMed
    1. Chang J., Tang B., Chen Y., Xia X., Qian M., Yang H. Two IncHI2 plasmid-mediated colistin-resistant Escherichia coli strains from the broiler chicken supply Chain in Zhejiang province, China. J. Food Prot. 2020;83:1402–1410. - PubMed