Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022;60(6):384-391.
doi: 10.5114/reum.2022.123669. Epub 2022 Dec 30.

14-3-3η protein is associated with disease activity and osteoporosis in patients with rheumatoid arthritis

Yasmin Adel  1 , Yousra Sadeq  2
Affiliations

14-3-3η protein is associated with disease activity and osteoporosis in patients with rheumatoid arthritis

Yasmin Adel et al. Reumatologia. 2022.

Abstract

Introduction: This study was designed to explore the potential association of serum 14-3-3η protein level with disease activity and bone mineral density (BMD) in Egyptian patients with rheumatoid arthritis (RA). Patients were recruited from the outpatient clinic at Mansoura University Hospital.

Material and methods: One hundred eighty-eight patients with RA and 192 matched controls were enrolled. The rheumatoid arthritis activity parameters were evaluated in RA patients. Bone mineral density was measured. Serum levels of 14-3-3η protein and IL-6 were estimated for all participants by enzyme-linked immunosorbent assays (ELISA).

Results: Rheumatoid arthritis patients had a significantly higher median serum 14-3-3η protein level compared to matched controls (p ≤ 0.05). Serum level of 14-3-3η protein was significantly correlated with DAS28-ESR (p ≤ 0.05) and serum IL-6 level (p ≤ 0.05). The rheumatoid arthritis-osteoporosis group had significantly higher serum 14-3-3η protein than the RA-osteopenia group and RA-control group. Similarly, the difference of the serum 14-3-3η protein between the RA-osteopenia group and the RA-control group was significant. In the linear regression analysis, the strongest factors that were associated with BMD in RA patients were the serum level of 14-3-3η protein (p ≤ 0.05), IL-6 (p ≤ 0.05) and DAS28-ESR (p ≤ 0.05).

Conclusions: Serum level of 14-3-3η protein was significantly elevated in RA patients compared to controls and is significantly correlated with parameters of activity disease. The RA-osteoporosis group had significantly higher serum 14-3-3η protein than the RA-osteopenia group and RA-control group. Serum 14-3-3η protein can be a promising biomarker to reflect RA activity and predict presence of osteoporosis in RA patients.

Keywords: 14-3-3η protein; diseases activity score; osteoporosis; rheumatoid arthritis.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Comparison of the serum 14-3-3η protein level between rheumatoid arthritis patients and controls.
Fig. 2
Fig. 2
Comparison of the serum 14-3-3η protein level among the rheumatoid arthritis-control, rheumatoid arthritis-osteopenia, and rheumatoid arthritis-osteoporosis groups.
See this image and copyright information in PMC

References

    1. Smolen JS, Aletaha D, McInnes IB. Rheumatoid arthritis. Lancet 2016; 388: 2023–2038, DOI: 10.1016/S0140-6736(16)30173-8. - DOI - PubMed
    1. Tanaka Y. Rheumatoid arthritis. Inflamm Regen 2020; 40: 20, DOI: 10.1186/s41232-020-00133-8. - DOI - PMC - PubMed
    1. Cau Y, Valensin D, Mori M, et al. Structure, function, involvement in diseases and targeting of 14-3-3 proteins: an update. Curr Med Chem 2018; 25: 5–21, DOI: 10.2174/0929867324666170426095015. - DOI - PubMed
    1. Sluchanko NN, Gusev NB. Moonlighting chaperone-like activity of the universal regulatory 14-3-3 proteins. FEBS J 2017; 284: 1279–1295, DOI: 10.1111/febs.13986. - DOI - PubMed
    1. Gogl G, Tugaeva KV, Eberling P, et al. Hierarchized phosphotarget binding by the seven human 14-3-3 isoforms. Nat Commun 2021; 12: 1677, DOI: 10.1038/s41467-021-21908-8. - DOI - PMC - PubMed