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. 2023 Jan;38(1):110-119.
doi: 10.1007/s12291-022-01041-z. Epub 2022 Apr 30.

Association of Angiotensin II Type 1 Receptor (AT1R) Gene Polymorphism with Angiotensin II Serum Levels in Patients with Essential Hypertension

M Prasad  1 , D Rajarajeswari  1 , K Ramlingam  1 , R Viswakumar  1 , B Suneel  1 , Jyothi Conjeevaram  2 , P Aruna  3 , Nusrath Fathima  4 , Sandeep Kumar Vishwakarma  4 , Aleem Ahmed Khan  4
Affiliations

Association of Angiotensin II Type 1 Receptor (AT1R) Gene Polymorphism with Angiotensin II Serum Levels in Patients with Essential Hypertension

M Prasad et al. Indian J Clin Biochem. 2023 Jan.

Abstract

Essential hypertension (EH) is a multifactorial, polygenic condition, and is one of the most important comorbidities that contributes to stroke, myocardial infarction, cardiac failure, and renal failure. The continuous increasing rate of morbidity and mortality associated with EH presents an unmet need of population-based studies to explore pathophysiology as well as newer strategies for better diagnosis, prognosis and treatment. This study aimed to determine genotype and allele frequencies of A1166C polymorphism of AT1R gene in Indian patients with EH and correlated with serum levels of Angiotensin II. A total of 200 patients with EH and 200 age- and gender-matched control individuals were included in this study from the General Medicine Department Outpatient at Narayana Medical College and Hospital, Nellore, Andhra Pradesh, India. Patients with systolic blood pressure (SBP) ≥ 140 mmHg and/or diastolic blood pressure (DBP) ≥ 90 mmHg were considered as hypertensive. The findings of this study revealed significantly increased risk of C/A heterozygote and allele C in both men and women. Moreover, both men and women patients with EH showed higher serum levels of Angiotensin II with C/A as well as AA genotypes. These findings indicate a significant association of 1166 C/A polymorphism of the AT1R gene with increased risk of hypertension in Indian population.

Keywords: 1166 C/A single nucleotide polymorphism; Angiotensin II; Essential hypertension; Genetic association; PCR–RFLP.

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Conflict of interest statement

Conflict of interestAll authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
A Identification of amplified AT1R gene on agarose gel in different samples through ethidium bromide staining B Identification of different genotypes of A1166C polymorphism of AT1R gene through agarose gel electrophoresis after PCR–RFLP
Fig. 2
Fig. 2
Overall and gender-wise genotype correlation analysis showing serum levels of Angiotensin II in patients with EH and controls (***p = 0.0001)
Fig. 3
Fig. 3
Heat map showing correlation of serum Angiotensin II levels between patients and controls with different genotypes. Each heat map represents Pearson's correlation coefficient value (r) within each block of heat map of A total study participants B men participants and C women participants. The r value towards + 1 indicates a strong positive relationship, − 1 indicates a strong negative relationship, and 0 indicates no relationship
See this image and copyright information in PMC

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