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. 2023 Jan 6:16:1106904.
doi: 10.3389/fnins.2022.1106904. eCollection 2022.

Comparison of the accuracy of axial length measurement by different imaging methods in Sprague Dawley rats

Yajun Wu  1   2   3 , Xiangdong Luo  4 , Yuliang Feng  1   2   3 , Jiasong Yang  1   2   3 , Hua Fan  2   3 , Xiaobo Cen  5 , Wensheng Li  2   3   1
Affiliations

Comparison of the accuracy of axial length measurement by different imaging methods in Sprague Dawley rats

Yajun Wu et al. Front Neurosci. .

Abstract

Background: Obtaining accurate axial length (AL) is very important for the establishment of animal models of myopia. The purpose of this study is to compare the accuracy of Quantel A-B scan, OD-1 A scan, and vernier caliper in measuring AL in Sprague Dawley (SD) rats.

Methods: In total, 60 5-week-old SD rats were divided into female rat group (n = 30) and male rat group (n = 30). Quantel A-B scan and OD-1 A scan were, respectively, used to measure the AL of both eyes of each living rat, and vernier caliper was used to measure the anterior-posterior diameter of each rat's eyeball. Besides, the correlation between refractive error (RE) and AL measured by different instruments was evaluated, and the accuracy of the three measurement methods was compared according to gender and left/right eyes.

Results: There were significant differences in AL and diopter of SD rats at the same age (p < 0.05). the AL of male rats was greater than that of female rats, while diopter (D) was the opposite; There was no significant difference in AL and D between left and right eyes in the same SD rats (p > 0.05); There were statistical differences among the three measurement methods (p < 0.05), AL measured by vernier caliper was the largest, followed by Quantel A-B scan, OD-1 A scan; Difference in AL between male and female was not statistically significant between the results obtained by Quantel A-B scan and vernier caliper (p > 0.05), but there were statistically significant differences between the other two measurement methods (p < 0.05).

Conclusion: Sex is the influencing factor of AL and RE. Imaging measurement can accurately measure the AL in living small rodents. Compared with OD-1 A scan, Quantel A-B scan may be more accurate.

Keywords: OD-1 A scan; Quantel A-B scan; axial length; measuring method; vernier caliper.

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Conflict of interest statement

XC was employed by WestChina-Frontier PharmaTech Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Comparison results of the mean values of three AL measurement methods. F-R, female group right eye; M-R, male group right eye; F-L, female group left eye; M-L, male group left eye; AL, axial length.
FIGURE 2
FIGURE 2
Comparison of AL in Sprague Dawley (SD) rats of different sex and eye types. (A) AL results from Quantel A-B Scan. (B) AL results from Vernier caliper. (C) AL results from OD-1 A scan. F-R, female group right eye; M-R, male group right eye; F-L, female group left eye; M-L, male group left eye; AL, axial length; ns indicates no statistical significance; *p < 0.05; ****p < 0.01.
FIGURE 3
FIGURE 3
Comparison of RE in Sprague Dawley (SD) rats of different sexes and eye types. F-R, female group right eye; M-R, male group right eye; F-L, female group left eye; M-L, male group left eye; RE, refractive errors; D, diopter; ns indicates no statistical significance; ****p < 0.01.
FIGURE 4
FIGURE 4
Comparison of AL difference between male and female rats by three different measurement methods. (A) AL difference in right eye between male and female rats. (B) AL difference in left eye between male and female rats. AL, axial length; R, right eye; L, left eye; M-F, AL difference between male and female rats; ns indicates no statistical significance; *p < 0.05; ***p < 0.01.
FIGURE 5
FIGURE 5
Correlation analysis between AL and D measured in different ways. (A) Correlation between AL and D measured by Quantel A-B scan. (B) Correlation between AL and D measured by Vernier caliper. (C) Correlation between AL and D measured by OD-1 A scan. AL, axial length; D, diopter; r2, Pearson correlation coefficient; p < 0.0001 indicates that there is great statistical significance.
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