Accelerated biodecolorization and detoxification of synthetic textile dye Acid Maroon V by bacterial consortium under redox mediator system

Abstract

The treatment of textile industrial wastewater is an important concern owing to its negative impact on the biosphere. The present study highlighted dye decolorization potential of bacterial consortium EDPA containing Enterobacter dissolvens AGYP1 and Pseudomonas aeruginosa AGYP2 in the presence of redox mediators. Rapid decolorization of Acid Maroon V (100 mg l^-1) was achieved in the presence of lawsone compared to other redox mediators. The dye decolorization was best fitted with first order kinetics with higher reaction kinetics (k1 = 0.328 h^-1) and regression coefficient (R² = 0.979). The removal of dye by the consortium was 1.47 times faster in 8 h with 0.01 mM lawsone. The consortium EDPA was able to decolorize 1200 mg l^-1 concentration of dye with apparent R _max , K _m and R _max /K _m values 1000 mg l^-1 h^-1, 5000 mg l^-1 and 0.2 h^-1, respectively. The lawsone-mediated system could decolorize the dye 80.44% in 10 h at the end of 11 dye spiking cycle. The superior biodecolorization of 14 different textile dyes was obtained in the presence of lawsone-mediated system. The intracellular enzyme activities of azoreductase, NADH-DCIP reductase, laccase, manganese peroxidase and lignin peroxidase increased significantly. The sequential microaerophilic-aerobic incubation resulted into 89.31% reduction of total aromatic amines. The microbial toxicity, phytotoxicity and genotoxicity measurements revealed biotransformation of toxic nature of dye Acid Maroon V into non-toxic metabolites by the action of consortium EDPA, and thus its suitability for biotreatment of dye containing industrial effluents.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-023-03466-6.

Keywords: Acid Maroon V; Consortium EDPA; Decolorization; Lawsone; Toxicity.

Fig. 1

Time profile of biodecolorization of Acid Maroon V by consortium EDPA in the presence of redox mediators

Fig. 2

Effect of lawsone concentration on decolorization of Acid Maroon V by consortium EDPA. Values are mean of three experiments ± SEM, compared by One-Way ANOVA with p ≤ 0.05

Fig. 3

Effect of dye concentration on Acid Maroon V decolorization by consortium EDPA. Comparison of dye removal in the absence and presence of under lawsone (A), double reciprocal plot for kinetic analysis of Acid Maroon V decolorization by consortium EDPA (B). Values are mean of three experiments ± SEM, compared by Two-Way ANOVA with p ≤ 0.05

Fig. 4

Repeated decolorization of Acid Maroon V by consortium EDPA under lawsone-mediated system. Values are mean of three experiments ± SEM, compared by One-Way ANOVA with p ≤ 0.05

Fig. 5

Biodecolorization of Acid Maroon V by consortium EDPA: UV–visible spectrum of dye decolorization (A); real image of decolorization of dye—C is control, ST is static condition, SH is shaking condition (B)

Fig. 6

Fate of total aromatic amines (TAA) and dye Acid Maroon V by bacterial consortium EDPA under sequential microaerophilic-aerobic condition. Values are mean of three experiments ± SEM, compared by One-Way ANOVA with p ≤ 0.05

Fig. 7

Genotoxicity analysis of untreated and treated dye samples on human peripheral blood lymphocytes: Extent of DNA damage in arbitrary units (A), percent cell distribution in various DNA damage classes (B), and representative comet images (C): control (a), dye (b), intermediate (static) (c), product (shaking) (d) and H₂O₂ (e); Values are mean of three experiments ± SEM, compared by One-Way ANOVA with ** p ≤ 0.01, *** p ≤ 0.001