Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jan 5:13:977487.
doi: 10.3389/fneur.2022.977487. eCollection 2022.

A protocol for the integration of multi-omics bioinformatics: Mechanism of acupuncture as an adjunctive therapy for alcohol use disorder

Peiming Zhang  1 , Xiaochang Lan  2 , Baochao Fan  1 , Yiming Chen  1 , Xiaojing Wei  1 , Xiangli Li  3 , Ni Fan  2 , Chunzhi Tang  1 , Liming Lu  1
Affiliations

A protocol for the integration of multi-omics bioinformatics: Mechanism of acupuncture as an adjunctive therapy for alcohol use disorder

Peiming Zhang et al. Front Neurol. .

Abstract

Background: Alcohol use disorder (AUD) has become a significant global factor in various diseases. As a non-pharmacological therapy, certain therapeutic potential has been found in acupuncture; however, in-depth mechanistic studies related to acupuncture for patients with AUD are still insufficient.

Methods: Based on a randomized control design and a multi-omics analysis plan, this protocol details the recruitment (42 AUD patients), group allocation (21 in acupuncture group vs. 21 in sham acupuncture group), intervention and follow-up (replacement drugs as a normal treatment, 2 weeks acupuncture duration, and 3 month follow-up), and data collection and analytical processes. For the clinical outcomes, in addition to the time required for alcohol withdrawal symptoms to subside as the primary outcome, changes in the alcohol withdrawal symptoms, alcohol craving, mood dysfunction, sleep disorder, fatigue, self-efficacy, gastrointestinal symptoms, the quality of life, and the relapse outcomes will be compared between the groups to confirm the acupuncture clinical effectiveness on alcohol withdraw. The gut microbiome and the fecal metabolomics will also be assessed to explore the association of the structure and the function of gut microflora and the mediation of acupuncture effect on AUD fully utilizing gut microflora multi-modal data and clinical information, via the combination of multi-omics methods, feature screening algorithms and appropriate models.

Discussion: The results of this study may help to strengthen clinical evidence of the mechanism of acupuncture intervention in patients with AUD, through understanding of the regulatory mechanism of acupuncture in the gut microbiome and its metabolism as well as AUD-related clinical manifestations.

Trial registration: Chinese Clinical Trial Registry ChiCTR2200058120. Registered on 24 Mar 2022.

Keywords: Jin's three-needle technique; acupuncture; alcohol use disorder; metabolomics; microbiome; multi-modal bioinfomation; multi-omics.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Flow diagram of the study process. *Sample collection 0, 1, 2 means to collect 10 fecal samples from each patient group (N1 and N2) at baseline and at the end of the last intervention; additionally, to collect 10 fecal samples from the health control group (N0). (A–N) means the 14 measurement tools applied in this study, distributed in specific study phases. (A) CIWA-Ar, Clinical institute alcohol withdrawal syndrome scale. (B) abs-VAS, Abstinence visual analog-rating scale. (C) AUQ, Alcohol urge questionnaire. (D) cue-VAS, Cue-related visual analog-rating scale. (E) BDI-II, Beck depression inventory II. (F) BAI, Beck anxiety inventory. (G) PSQI, Pittsburgh sleep quality index. (H) MFS, Mental fatigue scale. (I) GSES, General self-efficacy scale. (J) Gastrointestinal symptom rating scale. (K) QOL-DA, Quality of life scale for patients with drug addiction/dependence. (L) TLFB, Timeline followback. (M) AUDIT, Alcohol use disorder identification test. (N) MAST, Michigan alcohol screening test.
Figure 2
Figure 2
JTN acupoint groups (Sishen-zhen, Dingshen-zhen, and Shouzhi-zhen).
Figure 3
Figure 3
Different types of appliance (left: non-hollow patch for sham acupuncture; right: hollow patch for real acupuncture).
Figure 4
Figure 4
Acupuncture insertion angles.
See this image and copyright information in PMC

References

    1. Forouzanfar MH, Afshin A, Alexander LT, Anderson HR, Bhutta ZA, Biryukov S, et al. . Global, regional, and national comparative risk assessment of 79 behavioural, environmental and occupational, and metabolic risks or clusters of risks, 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet. (2016) 388:1659–724. 10.1016/S0140-6736(16)31679-8 - DOI - PMC - PubMed
    1. Shield K, Manthey J, Rylett M, Probst C, Wettlaufer A, Parry CDH, et al. . National, regional, and global burdens of disease from 2000 to 2016 attributable to alcohol use: a comparative risk assessment study. Lancet Public Heal. (2020) 5:e51–61. 10.1016/S2468-2667(19)30231-2 - DOI - PubMed
    1. Murray CJL, Aravkin AY, Zheng P, Abbafati C, Abbas KM, Abbasi-Kangevari M, et al. . Global burden of 87 risk factors in 204 countries and territories, 1990–2019: a systematic analysis for the Global Burden of Disease Study 2019. Lancet. (2020) 396:1223–49. 10.1016/S0140-6736(20)30752-2 - DOI - PMC - PubMed
    1. Rumgay H, Shield K, Charvat H, Ferrari P, Sornpaisarn B, Obot I, et al. . Global burden of cancer in 2020 attributable to alcohol consumption: a population-based study. Lancet Oncol. (2021) 22:1071–80. 10.1016/S1470-2045(21)00279-5 - DOI - PMC - PubMed
    1. Manthey J, Shield KD, Rylett M, Hasan OSM, Probst C, Rehm J. Global alcohol exposure between 1990 and 2017 and forecasts until 2030: a modelling study. Lancet. (2019) 393:2493–502. 10.1016/S0140-6736(18)32744-2 - DOI - PubMed