Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Oct-Dec;13(4):315-321.
doi: 10.4103/ccd.ccd_276_21. Epub 2022 Nov 3.

Correlation of Carbonic Anhydrase VI Enzyme, Total Proteins, Antioxidant Levels of Saliva and Dental Caries in Caries-Free and Caries-Active Children - A Case-Control Study

Anjana Cherath Vasudevan  1 , Nekkanti Sridhar  1 , Vinutha Bhat  2 , Meghana Bhat  2
Affiliations

Correlation of Carbonic Anhydrase VI Enzyme, Total Proteins, Antioxidant Levels of Saliva and Dental Caries in Caries-Free and Caries-Active Children - A Case-Control Study

Anjana Cherath Vasudevan et al. Contemp Clin Dent. 2022 Oct-Dec.

Abstract

Background and objectives: Factors in the biofilm influence the induction and advancement of the carious process. This study was done to relate and assess the levels of enzyme carbonic anhydrase VI, total protein, and antioxidants (superoxide dismutase [SOD], catalase, lipid peroxidase, and uric acid) in caries-free and caries-active children.

Methods: This case-control study comprised 60 children of age group 6-12 years who were evaluated for decayed missing filled teeth (dmft)/DMFT criteria and distributed into two groups: Group 1 - caries active (case) and Group 2 - caries free (control) for saliva collection with 30 participants in each of the above groups. Stimulated saliva was obtained, and the samples were then evaluated using biochemical lab tests. The data were then statistically evaluated using independent t-test.

Results: Catalase in the caries-free group was significantly higher, but the concentration of carbonic anhydrase (CAVI) enzyme, total protein, and other antioxidant enzyme activity was enhanced in caries-active children in which uric acid demonstrated a statistically significant difference with higher levels in caries-active group.

Conclusion: There is an increased concentration of CAVI enzyme in caries-active group and total protein showed a linear relation with caries activity. Antioxidant parameters such as SOD and lipid peroxidase were increased with caries activity. Uric acid was significantly higher in the caries-active group, whereas catalase showed an indirect relation with dental caries. Significant variations in the levels of these parameters imply that the levels of these components of saliva can act as strong markers of caries status in children.

Keywords: Antioxidants; carbonic anhydrase enzyme; dental caries; proteins; saliva.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
ELISA microplate reader
Figure 2
Figure 2
Standard graph for human carbonic anhydrase VI concentration (pg/ml)
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Ship JA. Diabetes and oral health: An overview. J Am Dent Assoc. 2003;134(Spec No):4S–10S. - PubMed
    1. Kirschvink N, De Moffarts B, Lekeux P. The oxidant/antioxidant equilibrium in horses. Vet J. 2008;177:178–91. - PubMed
    1. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: Harms and benefits for human health. Oxid Med Cell Longev 2017. 2017 8416763. - PMC - PubMed
    1. Tóthová L, Kamodyová N, Červenka T, Celec P. Salivary markers of oxidative stress in oral diseases. Front Cell Infect Microbiol. 2015;5:73. - PMC - PubMed
    1. Kimoto M, Kishino M, Yura Y, Ogawa Y. A role of salivary carbonic anhydrase VI in dental plaque. Arch Oral Biol. 2006;51:117–22. - PubMed