Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023:2630:1-11.
doi: 10.1007/978-1-0716-2982-6_1.

Detection of miRNAs

Afrah Bawazeer  1 , David C Prince  2
Affiliations

Detection of miRNAs

Afrah Bawazeer et al. Methods Mol Biol. 2023.

Abstract

MicroRNAs (miRNAs) are small, noncoding RNAs that regulate gene expression. They play an important role in many biological processes including human diseases. However, miRNAs are challenging to detect due to their short sequence length and low copy number. A number of conventional (e.g., Northern blot, microarray, and RT-qPCR) and emerging (e.g., nanostructured materials and electrochemical methods) techniques have been developed to detect miRNA, each with their own strengths and weaknesses. Some of these techniques have been combined to detect miRNAs as disease biomarkers in point-of-care (POC) settings. Nonetheless, there is still potential for further innovation to facilitate the detection of miRNAs.

Keywords: Biomarkers; MiRNA detection; Point-of-care setting.

PubMed Disclaimer

References

    1. Dexheimer PJ, Cochella L (2020) MicroRNAs: from mechanism to organism. Front Cell Dev Biol 8:409 - DOI
    1. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116(2):281–297 - DOI
    1. Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136(2):215–233 - DOI
    1. Cissell KA, Deo SK (2009) Trends in microRNA detection. Anal Bioanal Chem 394(4):1109–1116 - DOI
    1. Lee RC, Feinbaum RL, Ambros V (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75(5):843–854 - DOI