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. 2023 Jan 14:2023:8242646.
doi: 10.1155/2023/8242646. eCollection 2023.

A-Kinase Anchor Protein 95 Is Involved in ERK1/2-Elk-1 Signal Transduction in Colon Cancer

Xiangyu Kong  1 , Putian An  2 , Junping Xu  2 , Wenzhi Liu  2 , Feng Lin  2 , Yulong Yang  1
Affiliations

A-Kinase Anchor Protein 95 Is Involved in ERK1/2-Elk-1 Signal Transduction in Colon Cancer

Xiangyu Kong et al. Anal Cell Pathol (Amst). .

Abstract

Objectives: To assess A-kinase anchor protein 95 (AKAP95), B-Raf, extracellular regulated protein kinases 1/2 (ERK1/2), and Elk-1 expression in colon cancer tissue, and characterize AKAP95 associations with B-Raf, ERK1/2, Elk-1, and colon cancer clinicopathological indices.

Methods: The immunohistochemistry streptavidin-perosidase (SP) method was used to determine protein expression levels in 64 colon cancer and 32 para-carcinoma tissue specimens.

Results: (1) Positive AKAP95 expression rates in colon cancer tissue were higher when compared with para-carcinoma tissue (92.19% vs. 59.38%, P < 0.05). Similar findings were determined for B-Raf (76.56% vs. 25%, P < 0.05), ERK1/2 (90.63% vs. 31.25%, P < 0.05), and Elk-1 levels (92.19% vs. 40.63%, P < 0.05). (2) No significant associations were identified between AKAP95, B-Raf, ERK1/2, and Elk-1 protein expression and degree of differentiation, histological type, and lymph node metastasis in colon cancer samples (P > 0.05); however, in The Cancer Genome Atlas and Gene Expression Omnibus datasets, AKAP95 was closely related to immune infiltration, and highly expressed AKAP95 was negatively associated with overall survival and relapse free survival rates in colon cancer patients. (3) Correlations were observed between AKAP95 and ERK1/2, AKAP95 and Elk-1, B-Raf and ERK1/2, B-Raf and Elk-1, and ERK1/2 and Elk-1 (all P < 0.05), but no correlation was observed between AKAP95 and B-Raf (P > 0.05).

Conclusions: AKAP95 may affect immune infiltration levels in colon cancer by participating in ERK1/2-Elk-1 signal transduction.

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Conflict of interest statement

The author(s) declare(s) that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Representative images showing AKAP95, B-Raf, ERK1/2, and ELK-1 expressions in colon cancer and para-carcinoma tissue (magnification = 10×). (a1) No AKAP95 expression in para-carcinoma tissue. (b1) AKAP95 showing some expression (− or ±) in colon cancer tissue. (c1) Lowly expressed AKAP95 (+) in colon cancer tissue. (d1) Moderately expressed AKAP95 (++) in colon cancer tissue. (e1) Highly expressed AKAP95 (+++) in colon cancer tissue. (d1) and (e1) AKAP95 expression not only in the nuclei, but also in the cytoplasm. (a2) No B-Raf expression in para-carcinoma tissue. (b2) B-Raf showing some expression (− or ±) in colon cancer tissue. (c2) Lowly expressed B-Raf (+) in colon cancer tissue. (d2) Moderately expressed B-Raf (++) in colon cancer tissue. (e2) Highly expressed B-Raf (+++) in colon cancer tissue. (c2)–(e2) B-Raf expression in the cytoplasm. (a3) No ERK1/2 expression in para-carcinoma tissue. (b3) ERK1/2 showing some expression (− or ±) in colon cancer tissue. (c3) Lowly expressed ERK1/2 (+) in colon cancer tissue. (d3) Moderately expressed ERK1/2 (++) in colon cancer tissue. (e3) Highly expressed ERK1/2 (+++) in colon cancer tissue. (c3) ERK1/2 mainly expression in the cytoplasm. (d3) and (e3) ERK1/2 expression in the cytoplasm and nucleus. (a4) No ELK-1 expression in para-carcinoma tissue. (b4) ELK-1 showing some expression (− or ±) in colon cancer tissue. (c4) Lowly expressed ELK-1 (+) in colon cancer tissue. (d4) Moderately expressed ELK-1 (++) in colon cancer tissue. (e4) Highly expressed ELK-1 (+++) in colon cancer tissue. (c4) and (d4) ELK mainly expression in the cytoplasm. (e4) ELK-1 expression in the cytoplasm and nucleus.
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References

    1. Clister T., Greenwald E. C., Baillie G. S., Zhang J. AKAP95 organizes a nuclear microdomain to control local cAMP for regulating nuclear PKA. Cell Chemical Biology . 2019;26(6):885–891.e4. doi: 10.1016/j.chembiol.2019.03.003. - DOI - PMC - PubMed
    1. Kong X., Zhang D., Zhuang W., et al. AKAP95 promotes cell cycle progression via interactions with cyclin E and low molecular weight cyclin E. American Journal of Translational Research . 2016;8(2):811–826. - PMC - PubMed
    1. Chen X., Kong X., Zhuang W., et al. Dynamic changes in protein interaction between AKAP95 and CX43 during cell cycle progression of A549 cells. Scientific Reports . 2016;6(1):21224–21224. doi: 10.1038/srep21224. - DOI - PMC - PubMed
    1. Chen R., Chen Y., Yuan Y., et al. CX43 and AKAP95 regulate G1/S conversion by competitively binding to cyclin E1/E2 in lung cancer cells. Thoracic Cancer . 2020;11(6):1594–1602. doi: 10.1111/1759-7714.13435. - DOI - PMC - PubMed
    1. Jiang H., Lu X., Shimada M., Dou Y., Tang Z., Roeder R. G. Regulation of transcription by the MLL2 complex and MLL complex-associated AKAP95. Nature Structural & Molecular Biology . 2013;20(10):1156–1163. doi: 10.1038/nsmb.2656. - DOI - PMC - PubMed

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