CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity
- PMID: 36693989
- DOI: 10.1038/s41587-023-01665-3
CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity
Comment on
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Precise transcript targeting by CRISPR-Csm complexes.Nat Biotechnol. 2023 Sep;41(9):1256-1264. doi: 10.1038/s41587-022-01649-9. Epub 2023 Jan 23. Nat Biotechnol. 2023. PMID: 36690762 Free PMC article.
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References
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- Lennox, K. A. & Behlke, M. A. Mini-review on current strategies to knockdown long non-coding RNAs. J. Rare Dis. Res. Treat. 1, 66–70 (2016). A review article that describes the limitations of RNAi, especially for knockdown of long non-coding RNAs. - DOI
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- Ai, Y., Liang, D. & Wilusz, J. E. CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells. Nucleic Acids Res. 50, e65 (2022). This paper reports the toxic side effects of Cas13’s trans-cleavage when used for RNA knockdown in eukaryotic cells. - DOI - PubMed - PMC
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