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Comment
. 2023 Sep;41(9):1204-1205.
doi: 10.1038/s41587-023-01665-3.

CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity

No authors listed
Comment

CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity

No authors listed. Nat Biotechnol. 2023 Sep.
No abstract available

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References

    1. Lennox, K. A. & Behlke, M. A. Mini-review on current strategies to knockdown long non-coding RNAs. J. Rare Dis. Res. Treat. 1, 66–70 (2016). A review article that describes the limitations of RNAi, especially for knockdown of long non-coding RNAs. - DOI
    1. Ai, Y., Liang, D. & Wilusz, J. E. CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells. Nucleic Acids Res. 50, e65 (2022). This paper reports the toxic side effects of Cas13’s trans-cleavage when used for RNA knockdown in eukaryotic cells. - DOI - PubMed - PMC
    1. Kolesnik, M. V. et al. Deciphering the most complex prokaryotic immune system. Biochemistry (Moscow) 86, 1301–1314 (2021). This review article summarizes the type III CRISPR immune system. - DOI - PubMed
    1. Staals, R. H. J. et al. RNA Targeting by the Type III-A CRISPR-Cas Csm Complex of Thermus thermophilus. Mol. Cell 56, 518–530 (2014). This paper characterizes the RNA targeting and cleavage activity of the Csm complex. - DOI - PubMed - PMC

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