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. 2023 Apr;45(4):437-450.
doi: 10.1007/s13258-022-01354-6. Epub 2023 Jan 25.

Comparative transcriptome analysis to reveal key ethylene genes involved in a Lonicera macranthoides mutant

YuQing Long  1   2 , Juan Zeng  1   2 , Min Yang  1   2 , XinRu Zhou  1   2 , Mei Zeng  1   2 , ChangYu Liu  3 , QiaoZhen Tong  1   2   4 , RiBao Zhou  5   6   7 , XiangDan Liu  8   9   10
Affiliations

Comparative transcriptome analysis to reveal key ethylene genes involved in a Lonicera macranthoides mutant

YuQing Long et al. Genes Genomics. 2023 Apr.

Abstract

Background: Lonicera macranthoides Hand.-Mazz. is an important medicinal plant. Xianglei-type (XL) L. macranthoides was formed after many years of cultivation by researchers on the basis of the natural mutant. The corolla of L. macranthoides XL remains unexpanded and its flowering period is nearly three times longer than that of wild-type (WT) plants. However, the molecular mechanism behind this desirable trait remains a mystery.

Objective: To understand the floral phenotype differences between L. macranthoides and L. macranthoides XL at the molecular level.

Methods: Transcriptome analysis was performed on L. macranthoides XL and WT. One DEG was cloned by RT-PCR amplification and selected for qRT-PCR analysis.

Results: Transcriptome analysis showed that there were 5603 differentially expressed genes (DEGs) in XL vs. WT. Enrichment analysis of DEGs showed that pathways related to plant hormone signal transduction were significantly enriched. We identified 23 key genes in ethylene biosynthesis and signal transduction pathways. The most abundant were the ethylene biosynthesis DEGs. In addition, the open reading frames (ORFs) of WT and XL ETR2 were successfully cloned and named LM-ETR2 (GenBank: MW334978) and LM-XL-ETR2 (GenBank: MW334978), respectively. qRT-PCR at different flowering stages suggesting that ETR2 acts in the whole stage of flower development of WT and XL.

Conclusions: This study provides new insight into the molecular mechanism that regulates the development of special traits in the flowers of L. macranthoides XL. The plant hormone ethylene plays an important role in flower development and flowering duration prolongation in L. macranthoides. The ethylene synthesis gene could be more responsible for the flower phenotype of XL. The genes identified here can be used for breeding and improvement of other flowering plants after functional verification.

Keywords: Ethylene; Flower; Lonicera macranthoides; Transcriptome; Xianglei.

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