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. 2022 Jul 1;16(3):157-163.
doi: 10.18502/ijhoscr.v16i3.10138.

Assessment the Effect of Human Umbilical Cord Wharton's Jelly Stem Cells on the Expression of Homing Genes: CXCR4 and VLA-4 in Cell Line of Prostate Cancer

Roya Asl Iranifam  1 , Masoud Maleki  1 , Vida Vahdani Kia  1 , Esmaeil Safavi  2 , Negin Sadi Khosroshahi  3
Affiliations

Assessment the Effect of Human Umbilical Cord Wharton's Jelly Stem Cells on the Expression of Homing Genes: CXCR4 and VLA-4 in Cell Line of Prostate Cancer

Roya Asl Iranifam et al. Int J Hematol Oncol Stem Cell Res. .

Abstract

Background : Prostate cancer is the second most common cancer in the male that affects the health, social and economic life of person. Different compounds such as Wharton's jelly, have been used to treat prostate cancer. Wharton's jelly is a tissue rich in cells with mesenchymal morphology. Wharton's jelly compound inhibited the growth of various cancer cells, including ovarian, osteosarcoma, breast, and prostate cancers, and also reduced the expression of CXCR4 and VLA-4 genes involved in the metastasis process. Materials and Methods: To do this research, Wharton's jelly stem cells and DU145 cancer cell line were cultured. After cell culture, the effect of Wharton's jelly on this cell line was evaluated by scratching and MTT assay. The expression of CXCR4 and VLA-4 genes was also evaluated by Real-time PCR. Results: The results of MTT and Scratching tests showed that Wharton's jelly inhibited the growth of DU145 cancer cells and also decreased the expression level of CXCR4 and VLA-4 genes. Conclusion: The results of this study showed that Wharton's jelly can be considered as an effective compound for decreasing metastasis of prostate cancer.

Keywords: CXCR4 gene; Prostate cancer; VLA-4 gene; Wharton jelly.

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Figures

Figure1
Figure1
Statistical analyzes showed a significant difference in the cell growth of Wharton’s jelly-treated cells compared to the control group at 30M and 15M concentrations. This indicated inhibition of DU145 cell growth by Wharton’s jelly (p value <0.05).
Figure 2
Figure 2
Cell migration at different doses of wharton’ s jelly (P <0.001)
Figure 3
Figure 3
The effect of 15%Wharton’s jelly on cancer cell migration (* 400)
Figure 4
Figure 4
The effect of 30% Wharton’s jelly on cancer cell migration (* 400)
Figure 5
Figure 5
Cell morphology. a: Wharton’s jelly-induced cells with apoptosis. b: DU145 cancer cells without induction. c: Wharton’s jelly stem cells (* 1000).
Figure 6
Figure 6
The expression of CXCR-4 and VLA-4 genes in the DU145 cell after treatment with Wharton ‘s jelly.
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