Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jan-Feb;39(1):133-138.
doi: 10.12669/pjms.39.1.5905.

Association of the BB genotype of the ABO gene with the risk of acute myocardial infarction in hospital-based study

Farzana Abubakar Yousuf  1 , Iqbal Azam  2 , Asal Khan Tareen  3 , Khawar A Kazmi  4 , Jibran Sualeh Muhammad  5 , Mohammad Perwaiz Iqbal  6
Affiliations

Association of the BB genotype of the ABO gene with the risk of acute myocardial infarction in hospital-based study

Farzana Abubakar Yousuf et al. Pak J Med Sci. 2023 Jan-Feb.

Abstract

Objectives: The ABO gene locus has been identified to be associated with myocardial infarction in patients with coronary heart disease. The primary focus of this hospital-based study was to explore the relationship of ABO blood groups and ABO genotypes with acute myocardial infarction (AMI) in Karachi, Pakistan.

Methods: In a comparative cross-sectional study, an equal number of adult AMI patients and healthy controls (n=275 in each group; age range 30-70 years, both males and females) were recruited from the Aga Khan University and NICVD, Karachi, with informed consent. The blood samples were analyzed for ABO blood groups and other biomarkers. PCR followed by RFLP techniques were employed for determining the ABO genotypes. Multinomial regression was used to evaluate the association of genotypes with the risk of AMI.

Results: Thirteen different combinations of ABO genotypes were observed while the O2O2 and A2A2 genotypes were not detected. No significant association based on the distribution of blood groups A, B, O and AB among AMI patients and healthy individuals was observed. The odds of AMI were 3.32 times in subjects with BB genotype as compared to subjects with OO genotypes after adjustment of age, gender, body mass index, heart rate, total cholesterol, and waist circumference [AOR (95% CI) =3.32 (1.36-8.08), p-value =0.008].

Conclusion: Our hospital-based study indicates that ABO genotype BB was significantly associated with the risk of AMI. This harmful effect of the BB genotype could have a possible relationship with AMI's development in the Pakistani population.

Keywords: ABO blood groups; ABO genotypes; Acute myocardial infarction; Association study; Pakistani population.

PubMed Disclaimer

Conflict of interest statement

Conflicts of Interests: None.

Figures

Fig.1
Fig.1
2% agarose gel showing the amplification of ABO gene (exons 6 and 7). The primer pairs mo46/57 which yields 252bp fragment and mo101/71 primer pair which yields 843bp fragment, Lane 1 shows 50-1000bp ladder, Lane 2-4 are the PCR amplification bands.
Fig.2
Fig.2
Digestion patterns of the BB genotypes using KpnI and HpaII restriction enzymes separated on a 4% agarose gel Lane 1 shows ΦX174 /HaeIII marker, Lane 2 shows BB genotype pattern having 309bp, 252bp, 223bp, 137bp and 119bp, fragments, Lane 3 shows 100bp marker.
See this image and copyright information in PMC

References

    1. WHO. [(Accessed on February 4, 2022)]. https: //www.worldlifeexpectancy.com/country-health-profile/pakistan.2022 .
    1. Jafary MH, Samad A, Ishaq M, Jawaid SA, Ahmad M, Vohra EA. Profile of acute myocardial infarction (AMI) in Pakistan. Pak J Med Sci. 2007;23(4):485–489.
    1. Yamamoto F, Cid E, Yamamoto M, Blancher A. ABO research in the modern era of genomics. Transfus Med Rev. 2012;26(2):103–118. doi:10.1016/j.tmrv.2011.08.002. - PubMed
    1. Chen Z, Yang SH, Xu H, Li JJ. ABO blood group system and the coronary artery disease:an updated systematic review and meta-analysis. Sci Rep. 2016;6:23250. doi:10.1038/srep23250. - PMC - PubMed
    1. Rehman GU. ABO and Rh(D) blood groups distribution in Pakistan:a systematic review. Forensic Res Criminal Int J. 2020;8(6):237–244. doi:10:15406/frcij:2020.08.00334.

LinkOut - more resources