Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Oct-Dec;14(4):69-74.
doi: 10.32607/actanaturae.11747.

Rhodobacter capsulatus PG Lipopolysaccharide Blocks the Effects of a Lipoteichoic Acid, a Toll-Like Receptor 2 Agonist

S V Zubova  1 , N I Kosyakova  2 , S V Grachev  1   3 , I R Prokhorenko  1
Affiliations

Rhodobacter capsulatus PG Lipopolysaccharide Blocks the Effects of a Lipoteichoic Acid, a Toll-Like Receptor 2 Agonist

S V Zubova et al. Acta Naturae. 2022 Oct-Dec.

Abstract

Lipopolysaccharides (LPS) and lipoteichoic acids (LTA) are the major inducers of the inflammatory response of blood cells caused by Gram-negative and some Gram-positive bacteria. CD14 is a common receptor for LPS and LTA that transfers the ligands to TLR4 and TLR2, respectively. In this work, we have demonstrated that the non-toxic LPS from Rhodobacter capsulatus PG blocks the synthesis of pro-inflammatory cytokines during the activation of blood cells by Streptococcus pyogenes LTA through binding to the CD14 receptor, resulting in the signal transduction to TLR2/TLR6 being blocked. The LPS from Rhodobacter capsulatus PG can be considered a prototype for developing preparations to protect blood cells against the LTA of gram-positive bacteria.

Keywords: CD14; Rhodobacter capsulatus; TLR; cytokines; lipopolysaccharide; lipoteichoic acid.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Effect of R. capsulatus PG LPS on TNF-α secretion upon activation of blood cells by E. coli LPS, S. enterica LPS, or S. piogenes LTA, n = 7. *p < 0.05
Fig. 2
Fig. 2
Effect of R. capsulatus PG LPS on IL-6 secretion upon activation of blood cells by E. coli LPS, S. enterica LPS, or S. piogenes LTA, n = 7. *p < 0.05
Fig. 3
Fig. 3
Effect of R. capsulatus PG LPS on IL-1β secretion upon activation of blood cells by E. coli LPS, S. enterica LPS, or S. piogenes LTA, n = 7. *p < 0.05
Fig. 4
Fig. 4
Effect of R. capsulatus PG LPS on IL-8 secretion upon activation of blood cells by E. coli LPS, S. enterica LPS, or S. piogenes LTA, n = 7. *p < 0.05
Fig. 5
Fig. 5
Effect of Ab CD14 on TNF-α secretion upon activation of blood cells by E. coli LPS, S. enterica LPS, S. pyogenes LTA, n = 7. *p < 0.05
See this image and copyright information in PMC

References

    1. Ginsburg L.. Lancet. Infect. Dis. 2002;2(3):171–179. - PubMed
    1. Zhang G., Meredith T.C., Kahne D.. Curr. Opin. Microbiol. 2013;16(6):779–785. - PMC - PubMed
    1. Schwandner R., Dziarski R., Wesche H., Rothe M., Kirschning C.J.. J. Biol. Chem. 1999;274(25):17406–17409. - PubMed
    1. Schroder N.W.J., Morath S., Alexander C., Hamann L., Hartung T., Zahringer U., Gobel U.B., Weber J.R., Schumann R.R., Biol. Chem. 2003;278(18):15587–15594. - PubMed
    1. Im J., Choi H.S., Kim S.K., Woo S.S., Ryu Y.H., Kang S.S., Yun C.H., Han S.H.. Cancer Lett. 2009;274(1):109–117. - PubMed

LinkOut - more resources