. 2023 Mar;33(3):258-261.

doi: 10.1038/s41422-023-00781-8. Epub 2023 Jan 25.

Repeated vaccination of inactivated SARS-CoV-2 vaccine dampens neutralizing antibodies against Omicron variants in breakthrough infection

Bo Gao^#^{1

2}, Liheng He^#¹, Yujie Bao^#³, Yingying Chen^#^{1

4

5}, Guanzhu Lu³, Yu Zhang⁶, Yingjie Xu⁶, Bing Su^{1

2

7}, Jie Xu⁸, Ying Wang^{9

10

11

12}, Leng-Siew Yeap^{13

14}

Affiliations

¹ Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
² Center for Immune-Related Diseases at Shanghai Institute of Immunology, Department of Endocrinology and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
³ Department of Infectious Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁴ Shanghai Key Laboratory of Emergency Prevention, Diagnosis and Treatment of Respiratory Infectious Diseases (20dz2261100), Shanghai, China.
⁵ Key Laboratory of Parasite and Vector Biology, Ministry of Health, School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁶ Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Department of Biochemistry and Molecular Cell Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁷ Shanghai Jiao Tong University School of Medicine-Yale Institute for Immune Metabolism, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁸ Department of Infectious Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. xujie@shsmu.edu.cn.
⁹ Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ywangssmu@shsmu.edu.cn.
¹⁰ Shanghai Key Laboratory of Emergency Prevention, Diagnosis and Treatment of Respiratory Infectious Diseases (20dz2261100), Shanghai, China. ywangssmu@shsmu.edu.cn.
¹¹ Key Laboratory of Parasite and Vector Biology, Ministry of Health, School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ywangssmu@shsmu.edu.cn.
¹² Shanghai Institute of Virology, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ywangssmu@shsmu.edu.cn.
¹³ Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China. yeaplengsiew@shsmu.edu.cn.
¹⁴ Center for Immune-Related Diseases at Shanghai Institute of Immunology, Department of Endocrinology and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. yeaplengsiew@shsmu.edu.cn.

^# Contributed equally.

PMID: 36697703
PMCID: PMC9875169
DOI: 10.1038/s41422-023-00781-8

Repeated vaccination of inactivated SARS-CoV-2 vaccine dampens neutralizing antibodies against Omicron variants in breakthrough infection

Bo Gao et al. Cell Res. 2023 Mar.

. 2023 Mar;33(3):258-261.

doi: 10.1038/s41422-023-00781-8. Epub 2023 Jan 25.

Authors

Bo Gao^#^{1

2}, Liheng He^#¹, Yujie Bao^#³, Yingying Chen^#^{1

4

5}, Guanzhu Lu³, Yu Zhang⁶, Yingjie Xu⁶, Bing Su^{1

2

7}, Jie Xu⁸, Ying Wang^{9

10

11

12}, Leng-Siew Yeap^{13

14}

Affiliations

¹ Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
² Center for Immune-Related Diseases at Shanghai Institute of Immunology, Department of Endocrinology and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
³ Department of Infectious Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁴ Shanghai Key Laboratory of Emergency Prevention, Diagnosis and Treatment of Respiratory Infectious Diseases (20dz2261100), Shanghai, China.
⁵ Key Laboratory of Parasite and Vector Biology, Ministry of Health, School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁶ Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Department of Biochemistry and Molecular Cell Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁷ Shanghai Jiao Tong University School of Medicine-Yale Institute for Immune Metabolism, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁸ Department of Infectious Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. xujie@shsmu.edu.cn.
⁹ Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ywangssmu@shsmu.edu.cn.
¹⁰ Shanghai Key Laboratory of Emergency Prevention, Diagnosis and Treatment of Respiratory Infectious Diseases (20dz2261100), Shanghai, China. ywangssmu@shsmu.edu.cn.
¹¹ Key Laboratory of Parasite and Vector Biology, Ministry of Health, School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ywangssmu@shsmu.edu.cn.
¹² Shanghai Institute of Virology, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ywangssmu@shsmu.edu.cn.
¹³ Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China. yeaplengsiew@shsmu.edu.cn.
¹⁴ Center for Immune-Related Diseases at Shanghai Institute of Immunology, Department of Endocrinology and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. yeaplengsiew@shsmu.edu.cn.

^# Contributed equally.

PMID: 36697703
PMCID: PMC9875169
DOI: 10.1038/s41422-023-00781-8

No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

**Fig. 1. Comparisons of pseudovirus neutralizing titers in BA.2-infected individuals with different vaccination backgrounds.**
a The schematic diagram shows the number of unvaccinated, 2-dose and 3-dose vaccinated individuals prior to BA.2 infection or breakthrough infection. b The stacked bars represent the proportion of all BA.2-infected patients, presenting with the indicated clinical symptoms. The numbers shown in the bar and above the bar represent the percentages of people with the indicated clinical symptoms and the total number of people in each vaccination group, respectively. Two-tailed χ² tests were performed in b. ****P < 0.0001; ns, not significant. c The percentages of individuals with the indicated pVNT₅₀ values in SARS-CoV-2, BA.2, BA.4 and BA.5 pseudovirus neutralization assays are displayed. d Box plots show the pVNT₅₀ values of samples that are above the detection limit. Each black dot represents a sample. The whiskers show the minimum and maximum pVNT₅₀ titers. The line in the box represents median. The GMTs of each group are indicated in numbers on the top of each box plot. Black dotted line represents the detection threshold. e The ratio of the GMTs of the 2-dose or 3-dose group over the unvaccinated group is shown. Orange numbers indicate the ratio of the GMT of the 3-dose group over the unvaccinated group. Green numbers indicate the ratio of the GMT of the 2-dose group over the unvaccinated group. Statistical analysis was performed using unpaired two-tailed Mann-Whitney tests. *P < 0.05; ns, not significant.

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Repeated vaccination of inactivated SARS-CoV-2 vaccine dampens neutralizing antibodies against Omicron variants in breakthrough infection

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Repeated vaccination of inactivated SARS-CoV-2 vaccine dampens neutralizing antibodies against Omicron variants in breakthrough infection

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