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. 2023 Jan 10:13:1070817.
doi: 10.3389/fmicb.2022.1070817. eCollection 2022.

Effects of nitrogen input on soil bacterial community structure and soil nitrogen cycling in the rhizosphere soil of Lycium barbarum L

Yuekun Li  1 , Nan Zou  2 , Xiaojie Liang  1 , Xuan Zhou  1 , Shuhan Guo  2 , Yajun Wang  1 , Xiaoya Qin  1 , Yehan Tian  2 , Jin Lin  2
Affiliations

Effects of nitrogen input on soil bacterial community structure and soil nitrogen cycling in the rhizosphere soil of Lycium barbarum L

Yuekun Li et al. Front Microbiol. .

Abstract

Lycium barbarum L., goji berry, is a precious traditional Chinese medicine and it is homology of medicine and food. Its growth is heavily dependent on nitrogen. The use of chemical fertilizers has significantly promoted the yield of goji berry and the development of the L. barbarum L. industry. However, crop plants are inefficient in the acquisition and utilization of applied nitrogen, it often leads to excessive application of nitrogen fertilizers by producers, which cause negatively impact to the environment ultimately. The exploration of an interaction model which deals with crops, chemical fertilizers, and rhizosphere microbes to improve nitrogen use efficiency, is, therefore, an important research objective to achieve sustainable development of agriculture greatly. In our study, we explored the effects of nitrogen input on soil microbial community structure, soil nitrogen cycling, and the contents of nutrients in L. barbarum fruits. The structure and composition of the bacterial community in the rhizosphere soil of L. barbarum were significantly different under different nitrogen supply conditions, and high nitrogen addition inhibited the diversity and stability of bacterial communities. Low nitrogen input stimulated the relative abundance of ammonia-oxidizing bacteria (AOB), such as Nitrosospira, catalyzing the first step of the ammonia oxidation process. The results of the GLMM model showed that the level of nitrogen fertilizer (urea) input, the relative abundance of AOB, the relative abundance of Bradyrhizobium, and their combinations had significant effects on the soil nitrogen cycling and contents of nutrients in L. barbarum fruits. Therefore, we believe that moderately reducing the use of urea and other nitrogen fertilizers is more conducive to improving soil nitrogen use efficiency and Goji berry fruit quality by increasing the nitrogen cycling potential of soil microorganisms.

Keywords: Lycium barbarum L.; bacterial community; goji berry quality; nitrogen cycle; rhizosphere soil.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Influences of experimental N addition on microbial function and nitrogen metabolism function.
Figure 2
Figure 2
Response of the levels of fruit metabolites of Lycium chinensis to experimental N addition based on widely targeted metabolomics analysis.
Figure 3
Figure 3
Influences of experimental N addition on microbial diversity index and community composition.
Figure 4
Figure 4
N-induced changes in bacterial community structure and biomarkers.
Figure 5
Figure 5
Influences of experimental N addition on nitrogen metabolism related genes.
Figure 6
Figure 6
Response of co-occurrence networks of soil microbial communities to experimental N addition.
See this image and copyright information in PMC

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