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. 2023 Jul;143(7):1299-1302.e3.
doi: 10.1016/j.jid.2022.12.021. Epub 2023 Jan 26.

Characterizing Dermal Transcriptional Change in the Progression from Sun-Protected Skin to Actinic Keratosis

Bonnie LaFleur  1 , Clara Curiel-Lewandrowski  2 , Edgar Tapia  3 , Joel Parker  4 , Lisa White  4 , H-H Sherry Chow  3 , Andrew P South  5
Affiliations

Characterizing Dermal Transcriptional Change in the Progression from Sun-Protected Skin to Actinic Keratosis

Bonnie LaFleur et al. J Invest Dermatol. 2023 Jul.
No abstract available

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Conflict of interest statement

Conflict of Interest Statement

The authors have no conflicts of interest to declare.

Figures

Figure 1:
Figure 1:. Spatial Profiling identifies the greatest transcriptional change in the cytokeratin negative compartment comparing sun-protected skin with actinic keratosis.
a: Digital Spatial Profiler identified regions of interest (ROI) highlights examples of areas of patient tissue sampled for transcriptional profiling. b: Differentially expressed genes identified using a pairwise FDR adjusted p-value of less than 0.05 and an overall t-test statistic of 0.05 highlights greater number DEGs (y-axis) in the cytokeratin negative compartment (CK-) comparing sun protected normal skin (SP) with actinic keratosis center (Cen). CK+ = cytokeratin positive compartment, Edge = actinic keratosis edge.
Figure 2:
Figure 2:. Pathway changes in the progression from sun-protected skin to actinic keratosis.
a: Biplots showing centered log-ratio (CLR) transformed reads comparing sun-protected and actinic keratosis samples. Individual samples are shown with three color and symbol combinations and the mean values are larger symbols on the PCA plane with the CLR standardized gene expression plotted. For CK-negative samples the SP and AK edge cluster together, and for CK-positive samples AK edge and center cluster together relative to the SP samples. The bar charts help interpret the directionality of the projections which can be inferred from the PCA plot but is simplified by the bar graphs. b: Graphical representation of transcriptional change moving through sun protected skin to actinic keratosis. Major pathways (identified using methodology described in Ben-Ari Fuchs et al., 2016) changing comparing each of the three compartments are indicated within the arrows representing the overall extent of transcriptional change, green arrows represent transcriptional change within the cytokeratin positive compartment while orange arrows represent the dermal (cytokeratin negative) compartment.
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