Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Oct 26;15(20):8167-76.
doi: 10.1093/nar/15.20.8167.

The stability of oligodeoxyribonucleotide duplexes containing degenerate bases

N N Anand  1 , D M Brown, S A Salisbury
Affiliations
Free PMC article

The stability of oligodeoxyribonucleotide duplexes containing degenerate bases

N N Anand et al. Nucleic Acids Res. .
Free PMC article

Abstract

Oligodeoxyribonucleotides containing N4-methoxycytosine (mo4C), N4-methoxy-5-methylcytosine (mo4m5C) and other base-analogues were synthesised and used to compare the stabilities of duplexes containing mo4C.A and mo4C.G base pairs with those containing normal and mismatch pairs. The Tm values and other thermodynamic parameters are recorded. The otherwise identical duplexes containing a mo4C.A and a mo4C.G base pair have closely similar stabilities to each other and to the corresponding duplexes containing normal base pairs, considerably greater than the stabilities of those containing mismatch pairs. Corresponding observations are recorded in dot-blot experiments using M13 cloned DNA carrying an insert complementary to the oligonucleotides; approximate Td values are given.

PubMed Disclaimer

References

    1. J Med Chem. 1966 Jan;9(1):101-5 - PubMed
    1. Nucleic Acids Res. 1986 Oct 10;14(19):7727-36 - PubMed
    1. J Med Chem. 1968 Jan;11(1):144-8 - PubMed
    1. J Chem Soc Perkin 1. 1968;15:1925-9 - PubMed
    1. J Mol Biol. 1973 Aug 5;78(2):301-19 - PubMed

Publication types

MeSH terms